4.7 Article

Quantitative Risk Assessment of Five Foodborne Viruses in Shellfish Based on Multiplex qPCR

Related references

Note: Only part of the references are listed.
Article Immunology

Effect of Water, Sanitation, Handwashing, and Nutrition Interventions on Enteropathogens in Children 14 Months Old: A Cluster-Randomized Controlled Trial in Rural Bangladesh

Jessica A. Grembi et al.

Summary: We evaluated the impact of water, sanitation, and handwashing (WSH) and nutrition interventions on enteropathogens in children in Bangladesh. WSH interventions were associated with fewer enteric viruses compared to controls. There was no difference in bacterial and protozoan pathogens for WSH or nutrition interventions.

JOURNAL OF INFECTIOUS DISEASES (2023)

Article Biotechnology & Applied Microbiology

Use of bentonite-coated activated carbon for improving the sensitivity of RT-qPCR detection of norovirus from vegetables and fruits: The ISO 15216-1:2017 standard method extension

Mengxuan Tang et al.

Summary: Produce-related foodborne outbreaks are increasing worldwide. A highly sensitive BCAC-RT-qPCR method was developed in this study for detecting norovirus in fruits and vegetables. The method is fast, sensitive, and efficient in extracting viruses, and is suitable for detecting low levels of foodborne viruses.

FOOD MICROBIOLOGY (2023)

Article Chemistry, Applied

Prioritization of vegetable-borne biological hazards in Argentina using a multicriteria decision analysis tool

Victoria Brusa et al.

Summary: In this study, a scientific-based risk ranking of foodborne pathogens transmitted by leafy green vegetables in Argentina was conducted. Cryptosporidum spp., Toxoplasma gondii, and Norovirus were identified as the highest risk pathogens. Additionally, further epidemiological studies are needed to determine the association between vegetables and diseases such as Norovirus.

FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL (2023)

Article Environmental Sciences

Integrating life cycle assessment with quantitative microbial risk assessment for a holistic evaluation of sewage treatment plant

Ankita Bhatt et al.

Summary: An integrated approach combining LCA and QMRA was used to assess a sewage treatment plant in India. The study found that high electricity consumption had the greatest environmental impact, while the impacts on human health were measured as 0.01 DALYs pppy. The QMRA model developed in the study determined the health risks associated with the reuse of treated water, with a cumulative health risk of 0.07 DALYs pppy.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Environmental Sciences

Reduction of human fecal markers and enteric viruses in Sydney estuarine waters receiving wet weather overflows

Warish Ahmed et al.

Summary: This study tracked the reduction of fecal indicator bacteria and enteric viruses in estuarine water after wet weather overflows in Sydney, Australia. The results showed that human fecal markers were detected in all surface water samples, while the detection frequency was slightly lower at the 1-meter depth. In contrast, culturable enterococci concentrations were within the gastrointestinal risk benchmark by day 2 and day 4 for the two sites, respectively.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Environmental Sciences

Current decontamination challenges and potentially complementary solutions to safeguard the vulnerable seafood industry from recalcitrant human norovirus in live shellfish: Quo Vadis?

Neil J. Rowan

Summary: Safeguarding the seafood industry is crucial to protect human health. However, eliminating enteric viruses, especially in live shellfish, is challenging. Current disinfection methods are effective against bacteria but have limited effectiveness against enteric viruses. Therefore, alternative non-thermal technologies and novel diagnostic approaches are needed to ensure the safety of shellfish. Risk management planning and real-time ecosystem monitoring are also important for mitigating potential contaminant threats.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Review Engineering, Environmental

Enteric pathogen reduction targets for onsite non-potable water systems: A critical evaluation

Michael A. Jahne et al.

Summary: In this work, the authors compare and synthesize the log10-reduction targets (LRTs) for pathogens in onsite non-potable water systems (ONWS) to inform the selection of pathogen LRTs. The results show that there were minor differences in LRTs for human enteric viruses and parasitic protozoa between 2017 and 2021 efforts, despite differences in pathogen characterization methods. The greatest differences were observed in stormwater due to the use of municipal wastewater data and different reference pathogens.

WATER RESEARCH (2023)

Article Immunology

Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine

Zhendi Yu et al.

Summary: This study aimed to produce the recombinant P protein of norovirus and examine its blocking effect. The engineered strains expressed the P protein of NoVs GII.4, which showed binding capacity to histo-blood group antigens (HBGAs) through SDS-PAGE and ELISA. Rabbits were immunized to obtain neutralizing antibodies, which were found to block the binding of the P protein and human norovirus (HuNoV) to HBGAs. The recombinant P protein can induce antibodies to block HuNoV and murine norovirus (MNV) invasion.

VACCINES (2023)

Article Food Science & Technology

Prevalence of Foodborne Viruses in Berries Harvested in Canada

Eva Chatonnat et al.

Summary: The prevalence of foodborne viruses (HuNoV GI, HuNoV GII, HAV, and HEV) in commercially-produced cranberries and wild blueberries harvested in Canada was evaluated. Results showed that out of 234 cranberry samples, only 3 were positive for HuNoV GI, and no HuNoV GII or HAV were detected. None of the 150 blueberry samples tested positive for HEV. Overall, the prevalence of foodborne viruses in these berries is low, making them relatively safe for consumption.

FOODS (2023)

Article Engineering, Environmental

Deterministic Effect of pH on Shaping Soil Resistome Revealed by Metagenomic Analysis

Zishu Liu et al.

Summary: Soil is the major reservoir of antibiotic resistance genes (ARGs), particularly multidrug efflux pump genes, which have raised concerns due to their high prevalence in natural soil ecosystems. This study examined the influence of soil pH on the selection and composition of multidrug efflux pump genes in soil resistomes. The findings indicate that acidic soil environments significantly enrich multidrug efflux pump genes, reflecting the benefits of high soil proton activity on these genes.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023)

Article Environmental Sciences

Comparative reductions of norovirus, echovirus, adenovirus, Campylobacter jejuni and process indicator organisms during water filtration in alluvial sand

Louise Weaver et al.

Summary: Sand filtration is a cost-effective method for reducing microbial pathogens in drinking water. This study investigated the removal of various pathogens in alluvial sand filters and found that the reduction of pathogens was mainly influenced by their isoelectric points. The findings also have important implications for sand filter design, risk assessments of drinking water supplies, and determining safe setback distances.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Multidisciplinary Sciences

Inter-bacterial mutualism promoted by public goods in a system characterized by deterministic temperature variation

Yuxiang Zhao et al.

Summary: Research shows that high temperature can promote mutualism and reduce competition among microbial communities. Genomic analysis and culturing experiments in a composting system with temperature variation (20°C-70°C) reveal that temperature selects for stress-tolerant strains (Thermobifida fusca and Saccharomonospora viridis), and mutualistic interactions emerge between them and other strains through the sharing of cobalamin.

NATURE COMMUNICATIONS (2023)

Article Immunology

Effect of Norovirus Inoculum Dose on Virus Kinetics, Shedding, and Symptoms

Yang Ge et al.

EMERGING INFECTIOUS DISEASES (2023)

Article Public, Environmental & Occupational Health

Quantitative Risk Assessments of Hepatitis A Virus and Hepatitis E Virus from Raw Oyster Consumption

Kriangsak Ruchusatsawat et al.

Summary: This study evaluated the quantitative risk of hepatitis A virus (HAV) and hepatitis E virus (HEV) from raw oyster consumption, proposing a HEV dose-response model. Results showed that consuming farm oysters poses a significantly higher risk of hepatitis A than hepatitis E.

RISK ANALYSIS (2022)

Article Environmental Sciences

Quantitative assessment of exposure to fecal contamination in urban environment across nine cities in low-income and lower-middle-income countries and a city in the United States

Yuke Wang et al.

Summary: The study using the SaniPath tool in ten cities showed that food pathways were the most common route of exposure to fecal contamination in low-income and lower-middle-income countries. Water pathways, such as open drains, flood water, and municipal drinking water, were site-specific and often limited to smaller geographic areas. Understanding the contribution of fecal exposure from multiple pathways, along with the contamination levels and contact frequencies for dominant pathways, could guide WASH programming and investments to reduce the risk of fecal contamination exposure.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

Article Engineering, Environmental

Human adenovirus in municipal solid waste leachate and quantitative risk assessment of gastrointestinal illness to waste collectors

Natalia Maria Lanzarini et al.

Summary: This study revealed the presence of infectious Human Adenovirus (HAdV) in leachate from waste management systems in Rio de Janeiro, Brazil. It highlights the potential health risk for waste collectors and the higher probability of gastrointestinal illness associated with inadvertent oral ingestion of the leachate.

WASTE MANAGEMENT (2022)

Article Engineering, Environmental

Statewide Quantitative Microbial Risk Assessment for Waterborne Viruses, Bacteria, and Protozoa in Public Water Supply Wells in Minnesota

Tucker R. Burch et al.

Summary: Infection risk from waterborne pathogens can be estimated using quantitative microbial risk assessment (QMRA). However, site-specific hazard identification and exposure assessment are often lacking in groundwater QMRAs, leading to poorly defined risks. This study estimated the infection risk for 9 waterborne pathogens based on a 2-year study in Minnesota, USA. The results showed variations in risk among different types of wells, with some exceeding the US benchmark. Additionally, while the overall risk was high, the average daily doses for individual pathogens were low.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

Article Food Science & Technology

Decontamination of SARS-CoV-2 from cold-chain food packaging provides no marginal benefit in risk reduction to food workers

Julia S. Sobolik et al.

Summary: This study evaluates the risk of SARS-CoV-2 infection among workers from contact with contaminated plastic packaging in a frozen food facility. The results show that standard infection control interventions, such as handwashing and masking, significantly reduce the risk. Additional decontamination of the packaging does not provide marginal benefits in risk reduction.

FOOD CONTROL (2022)

Article Public, Environmental & Occupational Health

Risk Assessment of Norovirus Illness from Consumption of Raw Oysters in the United States and in Canada

Regis Pouillot et al.

Summary: This study focuses on assessing the risk of norovirus infection and illness from consuming raw oysters, with the concentration of NoV in wastewater treatment plants identified as the most important factor influencing the risk for raw oyster consumers.

RISK ANALYSIS (2022)

Article Microbiology

Surveillance of Adenovirus and Norovirus Contaminants in the Water and Shellfish of Major Oyster Breeding Farms and Fishing Ports in Taiwan

Viji Nagarajan et al.

Summary: This study in Taiwan investigated the prevalence of AdVs and NoVs in water and shellfish samples, revealing a higher detection rate in the low-temperature period than in the high-temperature period. AdVs were more prevalent in water samples compared to shellfish samples, while NoVs were most commonly detected in water samples from fishing ports and market shellfish samples.

PATHOGENS (2022)

Article Microbiology

Detangling Seasonal Relationships of Fecal Contamination Sources and Correlates with Indicators in Michigan Watersheds

Amanda M. Wilson et al.

Summary: This study analyzed a dataset of 64 Michigan watersheds to gain insights into seasonal differences in fecal contamination sources, drivers, and chemical correlates. The results confirmed a link between human-specific sources (e.g., septic systems) and microbial water quality. The study also found that fate and transport mechanisms vary with seasonal hydrologic condition and microorganism source. Overall, this research contributes to the development of strategies to reduce the public health burden of surface water fecal contamination.

MICROBIOLOGY SPECTRUM (2022)

Article Virology

Detection and Molecular Characterization of Enteric Viruses in Bivalve Mollusks Collected in Arraial do Cabo, Rio de Janeiro, Brazil

Lilian Goncalves do Nascimento et al.

Summary: This study investigated the presence, seasonality, quantity, and genetic diversity of enteric viruses in bivalve samples. Norovirus, rotavirus A, human adenovirus, and human bocavirus were detected in the shellfish samples, with norovirus being the most common. These findings emphasize the importance of virological monitoring to ensure the quality and safety of shellfish and to monitor emerging viruses and novel variants.

VIRUSES-BASEL (2022)

Article Multidisciplinary Sciences

Assessment of rotavirus and norovirus emitted from water spray park: QMRA, diseases burden and sensitivity analysis

Hasan Pasalari et al.

Summary: This study used a quantitative microbial risk assessment model to estimate the health risk of two pathogenic viruses (rotavirus and norovirus) in the air of a recreational area. The annual mean concentrations of the viruses exceeded recommended values, indicating the need for mitigation actions to reduce the risk.

HELIYON (2022)

Article Food Science & Technology

Detection of norovirus, hepatitis A and hepatitis E viruses in multicomponent foodstuffs

Catherine Hennechart-Collette et al.

Summary: The research characterized a method derived from ISO standard to detect HAV, HEV and norovirus in artificially-contaminated multicomponent foodstuffs. Results showed no significant difference in recovery rates among settings and extraction yields varied for different viruses. LOD95 and LOQ values were determined for different types of viruses.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2021)

Article Environmental Sciences

Environmental risk assessment by using disability adjusted life year via constructing of a generalized linear model for morbidity estimation of waterborne pathogens

Tingting Gao et al.

Summary: The Environmental burden of disease (EBD) quantitatively evaluates the health impacts of pathogens using the disability-adjusted life year (DALY) method, and constructing a linear morbidity model can effectively compensate for missing data in DALY calculation, helping to optimize the process for EBD evaluation.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2021)

Review Food Science & Technology

Inactivation of Foodborne Viruses by High-Pressure Processing (HPP)

Alexander Govaris et al.

Summary: High-pressure processing (HPP) is an innovative non-thermal food preservation method that can effectively inactivate foodborne viruses with minimal impact on the physicochemical and sensory properties of foods. It has been found to be particularly effective in high-risk foods such as shellfish and vegetables.

FOODS (2021)

Article Environmental Sciences

Norovirus Extraction from Frozen Raspberries Using Magnetic Silica Beads

Philippe Raymond et al.

Summary: Human noroviruses (HuNoV) are a major cause of acute gastroenteritis worldwide, and frozen raspberries have been linked to multiple outbreaks. However, extracting HuNoV RNA from frozen raspberries remains challenging, with low recovery yields and inhibition of RT-qPCR limiting detection sensitivity. A new approach using magnetic silica beads showed relatively low recovery yields, highlighting the importance of reducing RT-qPCR inhibition to accurately assess HuNoV prevalence in frozen raspberries.

FOOD AND ENVIRONMENTAL VIROLOGY (2021)

Article Biotechnology & Applied Microbiology

One-step duplex RT-droplet digital PCR assay for the detection of norovirus GI and GII in lettuce and strawberry

Chongzhen Sun et al.

Summary: The study developed a sensitive RT-ddPCR method for detecting NoV GI and GII in lettuce and strawberry, showing lower limits of detection and higher recovery rates compared to RT-qPCR. This method is more reliable and sensitive for detecting foodborne viruses in low concentration samples.

FOOD MICROBIOLOGY (2021)

Article Food Science & Technology

Quantitative levels of norovirus and hepatitis A virus in bivalve molluscs collected along the food chain in the Netherlands, 2013-2017

Rene A. M. Dirks et al.

Summary: This study evaluated the contamination levels of norovirus and hepatitis A virus in oyster and mussel samples from 2013 to 2017. Results showed that around 53.1% of mussel samples tested positive for NoV RNA, while about 31.6% of oyster samples were positive for NoV RNA. It was also found that contamination levels of NoV in mussel samples collected from B-areas were significantly higher than in other areas.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2021)

Article Food Science & Technology

The European Union One Health 2020 Zoonoses Report

Summary: The report reviews zoonoses monitoring activities in EU Member States and non-member states in 2020, emphasizing the high reporting rates of campylobacteriosis and salmonellosis in humans and the detection of Salmonella in meat products. Additionally, the report provides updates on various diseases.

EFSA JOURNAL (2021)

Article Multidisciplinary Sciences

Etiological, epidemiological, and clinical features of acute diarrhea in China

Li-Ping Wang et al.

Summary: A national-based prospective surveillance of acute diarrhea patients in China between 2009 and 2018 revealed that patients aged <5 years mainly had viral infections, while patients aged 18-45 years were more likely to be infected by bacteria. This study provides key insights into the distribution of enteropathogens in China and highlights the importance of identifying predominant diarrheal pathogens for clinical diagnosis and targeted prevention measures.

NATURE COMMUNICATIONS (2021)

Article Microbiology

Human Fecal Pollution Monitoring and Microbial Risk Assessment for Water Reuse Potential in a Coastal Industrial-Residential Mixed-Use Watershed

Akechai Kongprajug et al.

Summary: The study investigated microbial contamination in coastal industrial-residential community areas, finding continuous fecal pollution mainly from human sewage. Strong correlations were observed among different microbial parameters, and samples were separated into two clusters based on land use type.

FRONTIERS IN MICROBIOLOGY (2021)

Article Food Science & Technology

Preliminary Quantitative Risk Assessment of Norovirus in Shellfish in the Yellow Sea and Bohai Sea of China

Feng Zhao et al.

Summary: A preliminary quantitative risk assessment was conducted to evaluate the health risk caused by norovirus in shellfish in the Yellow Sea and Bohai Sea of China. The results showed a low probability of illness caused by consuming shellfish contaminated with norovirus, but there were differences in infection factors among different age groups.

FOODBORNE PATHOGENS AND DISEASE (2021)

Article Food Science & Technology

Semi-quantitative food safety risk profile of the Australian red meat industry

Marta Hernandez-Jover et al.

Summary: The Australian red meat industry plays a significant role in the country's economy, with a high level of both domestic consumption and international trade. Constant reassessment of food safety risks is crucial for maintaining a resilient industry sector.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2021)

Article Immunology

Viral intra-host evolution in immunocompetent children contributes to human norovirus diversification at the global scale

Kentaro Tohma et al.

Summary: The study revealed that GII.4 noroviruses exhibit dynamic intra-host variation, while non-GII.4 viruses show minimal variation within hosts. The genetic variation within children explains the source of norovirus mutations that lead to diversification at the global scale.

EMERGING MICROBES & INFECTIONS (2021)

Article Environmental Sciences

Detection of Human Adenovirus, Rotavirus, and Enterovirus in Tap Water and Their Association with the Overall Quality of Water in Karachi, Pakistan

Muhammad Rashid et al.

Summary: This study investigated the presence of enteric viruses in tap water in Karachi, Pakistan, finding that HAdV, hEV, and GARV were detected in 20%, 43%, and 23% of samples, respectively. The study also revealed overall quality deficiencies and bacterial contaminations in tap water, with no samples free from contamination. The efficiency of small-scale water treatment methods for virus removal was also assessed.

FOOD AND ENVIRONMENTAL VIROLOGY (2021)

Review Food Science & Technology

Chronic Gastrointestinal and Joint-Related Sequelae Associated with Common Foodborne Illnesses: A Scoping Review

Kristen Pogreba-Brown et al.

FOODBORNE PATHOGENS AND DISEASE (2020)

Article Environmental Sciences

Detection, Quantification, and Microbial Risk Assessment of Group A Rotavirus in Rivers from Uruguay

Viviana Bortagaray et al.

FOOD AND ENVIRONMENTAL VIROLOGY (2020)

Review Biotechnology & Applied Microbiology

Methods for detection of viable foodborne pathogens: current state-of-art and future prospects

Antonio C. G. Foddai et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity

Yifan Zhu et al.

MOLECULES (2020)

Article Public, Environmental & Occupational Health

Prevention of Hepatitis A Virus Infection in the United States: Recommendations of the Advisory Committee on Immunization Practices, 2020

Noele P. Nelson et al.

MMWR RECOMMENDATIONS AND REPORTS (2020)

Article Biotechnology & Applied Microbiology

Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR

Shin-Young Lee et al.

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2018)

Review Microbiology

Human norovirus transmission and evolution in a changing world

Miranda de Graaf et al.

NATURE REVIEWS MICROBIOLOGY (2016)