Journal
FOODS
Volume 12, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/foods12183406
Keywords
degree of polymerization; enzymatic hydrolysis; Fourier-transform infrared spectroscopy; mannanase; molecular weight distribution; oligosaccharides
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Konjac glucomannan (KGM) is a high-molecular-weight polysaccharide that can be depolymerized into oligomers with high antioxidant activities.
Konjac glucomannan (KGM) is a high-molecular-weight polysaccharide that was originally extracted from the corms (underground storage organs) of Amorphophallus konjac. KGM and its oligomers have been reported as dietary fibers that exhibit an array of health benefits. The depolymerization of KGM via enzymatic hydrolysis at different conditions gives products of low viscosity and can be used for coating materials in microencapsulation. In the present study, konjac glucomannan hydrolysates (KGMHs) were produced by enzymatic hydrolysis using commercial mannanase at pH 4.5 at 70 degrees C for 5-120 min, then KGMHs' molecular weight (M-w), Degree of Polymerization (DP) and their bioactivities were determined. A longer hydrolysis time resulted in KGMH of a lower DP. Oligoglucomannans (M-w < 10,000) could be obtained after hydrolysis for 20 min. The DP of KGMH rapidly decreased during an early stage of the hydrolysis (first 40 min); DP reached around 7 at the end of the hydrolysis. Antioxidant activities were determined by the DPPH radical scavenging and FRAP assays of KGMHs prepared at pH 4.5 and evaluated at pH 2.0-8.0 depending on pH. KGMH having lower M-w exhibited higher antioxidant activities. KGMHs having the smallest molecular weight (M-w = 419) exhibited the highest DPPH radical scavenging activity. M-w and pH have a greater impact on KGMHs' bioactivities which can be useful information for KGMHs as functional ingredients.
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