Journal
NATURE GENETICS
Volume 49, Issue 1, Pages 97-109Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ng.3723
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Funding
- Evelyn Trust
- NIHR Cambridge Biomedical Research Centre
- British Heart Foundation [FS/13/29/30024, RM/13/3/30159, FS/11/77/29327]
- British Heart Foundation [FS/13/29/30024, RG/17/5/32936] Funding Source: researchfish
- Medical Research Council [MC_PC_12009] Funding Source: researchfish
- Stroke Association [TSA2016/02] Funding Source: researchfish
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Marfan syndrome (MFS) is a heritable connective tissue disorder caused by mutations in FBN1, which encodes the extracellular matrix protein fibrillin-1. To investigate the pathogenesis of aortic aneurysms in MFS, we generated a vascular model derived from human induced pluripotent stem cells (MFS-hiPSCs). Our MFS-hiPSC-derived smooth muscle cells (SMCs) recapitulated the pathology seen in Marfan aortas, including defects in fibrillin-1 accumulation, extracellular matrix degradation, transforming growth factor-beta (TGF-beta) signaling, contraction and apoptosis; abnormalities were corrected by CRISPR-based editing of the FBN1 mutation. TGF-beta inhibition rescued abnormalities in fibrillin-1 accumulation and matrix metalloproteinase expression. However, only the noncanonical p38 pathway regulated SMC apoptosis, a pathological mechanism also governed by Krilppel-like factor 4 (KLF4). This model has enabled us to dissect the molecular mechanisms of MFS, identify novel targets for treatment (such as p38 and KLF4) and provided an innovative human platform for the testing of new drugs.
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