Journal
MICROBIAL GENOMICS
Volume 9, Issue 8, Pages -Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mgen.0.001076
Keywords
Statistical comparison; Short-read sequencing; Genomic Proficiency Test; Whole Genome Sequencing; Quality control; Pathogenic bacteria
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The global surveillance and outbreak investigation of antimicrobial resistance (AMR) is shifting from traditional biology to bioinformatics, thanks to developments in whole-genome sequencing (WGS) technologies, bioinformatics tools, and reduced costs. This study presents the results of the first iteration of the Genomic PT (GPT), which assessed laboratories' capacity to produce reliable WGS data. Most laboratories delivered high-quality sequence data, with only two laboratories identified as underperforming. The study highlights the importance of systematic quality control procedures to ensure reliable WGS data for surveillance and outbreak investigation.
The global surveillance and outbreak investigation of antimicrobial resistance (AMR) is amidst a paradigm shift from traditional biology to bioinformatics. This is due to developments in whole- genome- sequencing (WGS) technologies, bioinformatics tools, and reduced costs. The increased use of WGS is accompanied by challenges such as standardization, quality control (QC), and data sharing. Thus, there is global need for inter- laboratory WGS proficiency test (PT) schemes to evaluate laboratories' capacity to produce reliable genomic data. Here, we present the results of the first iteration of the Genomic PT (GPT) organized by the Global Capacity Building Group at the Technical University of Denmark in 2020. Participating laboratories sequenced two isolates and corresponding DNA of Salmonella enterica, Escherichia coli and Campylobacter coli, using WGS methodologies routinely employed at their laboratories. The participants' ability to obtain consistently good- quality WGS data was assessed based on several QC WGS metrics. A total of 21 laboratories from 21 European countries submitted WGS and meta- data. Most delivered high- quality sequence data with only two laboratories identified as overall underperforming. The QC metrics, N50 and number of contigs, were identified as good indicators for high- sequencing quality. We propose QC thresholds for N50 greater than 20 000 and 25 000 for Campylobacter coli and Escherichia coli, respectively, and number of contigs >200 bp greater than 225, 265 and 100 for Salmonella enterica, Escherichia coli and Campylobacter coli, respectively. The GPT2020 results confirm the importance of systematic QC procedures, ensuring the submission of reliable WGS data for surveillance and outbreak investigation to meet the requirements of the paradigm shift in methodology.
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