4.7 Review

Biomedical engineering approaches for the delivery of JAGGED1 as a potential tissue regenerative therapy

Related references

Note: Only part of the references are listed.
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Summary: The extracellular C2 domain of the Jagged/Serrate and Delta families of transmembrane ligands is important for optimal activation of the Notch receptor. Mutations affecting an N-glycosylation site in the C2 domain of JAGGED1 have been found in tumors, reducing the ligand's ability to activate Notch. Site-specific glycan analysis showed that a complex-type or high-mannose N-glycan at this site is required for full Notch activation. The presence of the N-glycan limits the orientation of JAG1 relative to the membrane, facilitating Notch binding.

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Summary: JAG1 gene encodes Jagged1 protein, mutations of which cause Alagille syndrome characterized by bile duct abnormalities. In this study, two homozygous JAG1 knockout iPSC lines were generated using CRISPR-Cas9 technology. These iPSC lines displayed similar pluripotency as the original line, providing a valuable resource for studying Jagged1 function in human development and pathology.

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