Journal
NATURE BIOTECHNOLOGY
Volume 34, Issue 5, Pages 556-561Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3505
Keywords
-
Categories
Funding
- US National Science Foundation [EEC-0813570, CBET-1134541, CBET-1067565]
Ask authors/readers for more resources
Anabolic metabolism can produce an array of small molecules, but yields and productivities are low owing to carbon and energy inefficiencies and slow kinetics(1,2). Catabolic and fermentative pathways, on the other hand, are carbon and energy efficient but support only a limited product range(3,4). We used carbon and energy-efficient non-decarboxylative Claisen condensation reactions and subsequent beta-reduction reactions, which can accept a variety of functionalized primers and functionalized extender units and operate in an iterative manner, to synthesize functionalized small molecules. Using different omega- and omega-1-functionalized primers and alpha-functionalized extender units in combination with various termination pathways, we demonstrate the synthesis of 18 products from 10 classes, including omega-phenylalkanoic, alpha,omega-dicarboxylic, omega-hydroxy, omega-1-oxo, omega-1-methyl, 2-methyl, 2-methyl-2-enolic and 2,3-dihydroxy acids, beta-hydroxy-omega-lactones, and omega-1-methyl alcohols.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available