Journal
NATURE BIOTECHNOLOGY
Volume 34, Issue 5, Pages 518-524Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3423
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Funding
- National Human Genome Research Institute of the National Institutes of Health [HG006321, HG007827, HG003703]
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A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current through the pore can now be decoded into a DNA sequence using an algorithm. In this Historical Perspective, we describe the key steps in nanopore strand-sequencing, from its earliest conceptualization more than 25 years ago to its recent commercialization and application.
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