Multi-Omics Revealed the Molecular Mechanism of Maize (Zea mays L.) Seed Germination Regulated by GA3

Maize is a valuable raw material for feed and food production. Healthy seed germination is important for improving the yield and quality of maize. However, the molecular mechanisms that regulate maize seed germination remain unclear. In this study, multi-omics was used to reveal the molecular mechanism of seed germination induced by gibberellin (GA) in maize. The results indicated that 25,603 genes were differentially expressed (DEGs) and annotated in the GO database, of which 2515 genes were annotated in the KEGG database. In addition, 791 mature miRNAs with different expression levels were identified, of which 437 were known in the miRbase database and 354 were novel miRNAs. Integrative analysis of DEGs and miRNAs suggested that carbohydrate, lipid, amino acid, and energy metabolisms are the primary metabolic pathways in maize seed germination. Interestingly, a lipid metabolism-related gene named ZmSLP was found to negatively regulate maize germination. We transformed this gene into Arabidopsis thaliana to verify its function. The results showed that the germination rate of transgenic Arabidopsis seeds was obviously decreased, and the growth of seedlings was weaker and slower than that of WT plants, suggesting that this gene plays an important role in promoting seed germination. These findings provide a valuable reference for further research on the mechanisms of maize seed germination.

Automated summary

Maize is a valuable raw material for feed and food production, and understanding the molecular mechanisms of seed germination is crucial for improving yield and quality. This study used multi-omics to investigate the molecular mechanism of gibberellin-induced seed germination in maize. The results revealed differentially expressed genes and miRNAs related to carbohydrate, lipid, amino acid, and energy metabolisms. A lipid metabolism-related gene, ZmSLP, was found to negatively regulate maize germination, which was confirmed through transformation experiments in Arabidopsis. These findings provide valuable insights into the mechanisms of maize seed germination.