Journal
NATURE BIOTECHNOLOGY
Volume 34, Issue 8, Pages 857-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3594
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Funding
- National Institute of Child Health and Human Development [T32-HD40127]
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- Helen Lyng White Fellowship
- Career Development Award from Human Frontier Science Program [00063/2012]
- National Science Foundation [1450824]
- Whitehall Foundation
- McKnight Foundation
- Klingenstein Foundation
- Simons Foundation [SCGB 325407SS]
- NIH [R01NS091335, R01EY024294]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1450824] Funding Source: National Science Foundation
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Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to similar to 1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.
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