Journal
NATURE
Volume 537, Issue 7622, Pages 644-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature19774
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Funding
- FEBS
- MRC UK
- MRC [MC_U105674180] Funding Source: UKRI
- Medical Research Council [MC_U105674180, 1601061, 1373852] Funding Source: researchfish
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Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIII2CIV (the respirasome)-a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 angstrom and 6.7 angstrom, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII2 at 7.8 resolution. All observed density can be attributed to the known 80 subunitsof the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain.
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