A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions

West Nile virus (WNV) causes skin lesions in farmed crocodiles leading to the depreciation of the value of their hides and significant economic losses. However, there is no commercially available vaccine designed for use in crocodilians against WNV. We tested chimeric virus vaccines composed of the non-structural genes of the insect-specific flavivirus Binjari virus (BinJV) and genes encoding the structural proteins of WNV. The BinJV/WNV chimera, is antigenically similar to wild-type WNV but replication-defective in vertebrates. Intramuscular injection of two doses of BinJV/WNV in hatchling saltwater crocodiles (Crocodylus porosus) elicited a robust neutralising antibody response and conferred protection against viremia and skin lesions after challenge with WNV. In contrast, mock-vaccinated crocodiles became viraemic and 22.2% exhibited WNV-induced lesions. This suggests that the BinJV/WNV chimera is a safe and efficacious vaccine for preventing WNV-induced skin lesions in farmed crocodilians.

Automated summary

This study tested a chimeric virus vaccine composed of non-structural genes from the insect-specific flavivirus Binjari virus and structural proteins from West Nile virus. The vaccine elicited a strong antibody response and protected hatchling saltwater crocodiles against viremia and skin lesions caused by West Nile virus. The results indicate that the BinJV/WNV chimera is a safe and effective vaccine for preventing skin lesions in farmed crocodilians.