Journal
NPJ BIOFILMS AND MICROBIOMES
Volume 9, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41522-023-00409-0
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In this study, the researchers developed an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics. They found that specific gut microbes and metabolites can rapidly and dose-dependently increase gut permeability, providing a powerful tool for investigating barrier functions.
The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
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