4.7 Article

Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study

Journal

JOURNAL OF CLINICAL MEDICINE
Volume 12, Issue 19, Pages -

Publisher

MDPI
DOI: 10.3390/jcm12196354

Keywords

keratoconus; metagenomics; validation-study; microbiota

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This study utilized the mNGS 16S rRNA sequencing method to investigate the ocular microbiota of keratoconus patients who had not undergone any surgical treatment. The findings suggest that alterations in the microbiota may play a role in the development of keratoconus.
In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naive keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.

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