☆ 4.6 Article

Real-Time Monitoring of Exosomes Secretion from Single Cell Using Dual-Nanopore Biosensors

ACS SENSORS (2023)

Related references

Note: Only part of the references are listed.

Review Chemistry, Multidisciplinary

Glass Capillary-Based Nanopores for Single Molecule/Single Cell Detection

Xin Guan et al.

Summary: A glass capillary-based nanopore (G-nanopore) with tapered tip and flexible surface modifications provides a powerful sensing platform for diverse biological measurements. It enables noninvasive single molecule and subcellular single cell detections and shows potential for cancer diagnosis and treatment applications. However, current bottlenecks limit its widespread use in clinical applications.

ACS SENSORS (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Electrochemical Single-Cell Protein Therapeutics Using a Double-Barrel Nanopipette

Xiao-Mei Shi et al.

Summary: Single-cell protein therapeutics has the potential to enhance our understanding of how a specific protein treats cells individually instead of averaging across populations. A double-barrel nanopipette was used in this study to address the challenge. The results showed that delivering protein DJ-1 into cells can upregulate antioxidant protein and protect neural cells from oxidative stress. This nanotool provides a perspective for future single-cell therapies involving different therapeutic modalities.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Spatiotemporally Resolved Protein Detection in Live Cells Using Nanopore Biosensors

Hongshuai Zhang et al.

Summary: This study developed a dual-nanopore biosensor for label-free and spatiotemporal monitoring of proteins in living cells. By using affinity-tunable peptide probes, the sensor demonstrated reversible and sensitive detection of protein kinase A (PKA) catalytic subunit, with the ability to effectively eliminate interferences from cell membrane resistance and coexisting species. The sensor was also applied successfully in monitoring dynamic PKA activity changes under different stimulations in single live cells.

ACS NANO (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Fluorescence Analysis of Circulating Exosomes for Breast Cancer Diagnosis Using a Sensor Array and Deep Learning

Yuyao Jin et al.

Summary: Emerging liquid biopsy methods, such as the breast cancer liquid biopsy system developed in this study, provide a promising noninvasive approach for detecting and classifying cancer cells based on fluorescence signals collected from cells and exosomes. The integration of a fluorescence sensor array and deep learning tool AggMapNet allows for accurate differentiation between normal and cancerous cells, as well as accurate prediction of breast cancer in plasma-derived exosomes.

ACS SENSORS (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Organic Molecular Probe Enabled Ionic Current Rectification toward Subcellular Detection of Glutathione with High Selectivity, Sensitivity, and Recyclability

Yi-Li Liu et al.

Summary: Single-cell interrogation using solid-state nanoprobes allows for understanding the connection between cellular behavior and heterogeneity. A generic ionic current rectification (ICR) single-cell methodology is developed based on the charge property of the organic molecular probe (OMP), enabling subcellular detection of glutathione (GSH) with high selectivity, sensitivity, and recyclability.

ACS SENSORS (2022)

Add to Collection

Article Chemistry, Analytical

Electrochemical Resistive-Pulse Sensing of Extracellular Vesicles

Rui Jia et al.

Summary: Extracellular vesicles have potential applications in cancer diagnostics and cell signaling. Electrochemical resistive-pulse sensing allows for direct detection and analysis of these vesicles, as well as monitoring the effects of therapeutic agents.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Chemistry, Analytical

Confined Nanopipet as a Versatile Tool for Precise Single Cell Manipulation

Ru-Jia Yu et al.

Summary: This technical note presents a nanopipet-based method for precise manipulation of single cells, allowing the extraction of femtoliter volumes of cytoplasm for analysis. It can identify unusual single cell behavior and enable multi-imaging in single living cells through the injection of exogenous components.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Chemistry, Analytical

Development of Dual-Nanopore Biosensors for Detection of Intracellular Dopamine and Dopamine Efflux from Single PC12 Cell

Tao Zhao et al.

Summary: This study presents a dual-nanopore biosensor for the detection of dopamine at the single-cell level. The biosensor allows rapid and specific detection of dopamine, as well as monitoring changes in dopamine concentration within individual cells.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Molecular Identification of Tumor-Derived Extracellular Vesicles Using Thermophoresis-Mediated DNA Computation

Yike Li et al.

Summary: The study introduces a novel DNA computation device mediated by thermophoresis for detecting tEVs, achieving high sensitivity and specificity in analysis, providing new opportunities for cancer diagnosis.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Add to Collection

Article Multidisciplinary Sciences

Identifying extracellular vesicle populations from single cells

Jonas M. Nikoloff et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Add to Collection

Article Multidisciplinary Sciences

Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9

Mathilde Mathieu et al.

Summary: This study tracks the intracellular trafficking of two proteins before their secretion in EVs, reporting the biogenesis and protein markers of EV subtypes: ectosomes budding from the plasma membrane as well as exosomes from late endosomes.

NATURE COMMUNICATIONS (2021)

Add to Collection

Article Cell Biology

Hypoxia-stimulated ATM activation regulates autophagy-associated exosome release from cancer-associated fibroblasts to promote cancer cell invasion

Lei Xi et al.

Summary: Studies have shown that oxidized ATM phosphorylates BNIP3 to induce autophagosome accumulation and exosome release in breast CAFs. In addition, oxidized ATM phosphorylates ATP6V1G1, leading to lysosomal dysfunction and facilitating exosome release through fusion with multi-vesicular bodies. This process ultimately stimulates non-canonical NF-kappa B signaling and enhances the invasive abilities of recipient breast cancer cells.

JOURNAL OF EXTRACELLULAR VESICLES (2021)

Add to Collection

Article Biology

Real-Time analysis of exosome secretion of single cells with single molecule imaging

Pengfei Zhang et al.

Summary: The exosome-mediated response can promote or restrain diseases by regulating intracellular pathways, making it an effective marker for diagnosis and therapeutic control at the single-cell level. Label-free single-molecule imaging approaches may become a real-time quantitative tool for analyzing single exosomes and related secretion behaviors of single living cells due to their extreme sensitivity.

BIOCELL (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Simultaneous Quantification of Vesicle Size and Catecholamine Content by Resistive Pulses in Nanopores and Vesicle Impact Electrochemical Cytometry

Xin-Wei Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Add to Collection

Review Multidisciplinary Sciences

The biology, function, and biomedical applications of exosomes

Raghu Kalluri et al.

SCIENCE (2020)

Add to Collection

Article Cell Biology

Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer

Mingli Han et al.

CELL DEATH & DISEASE (2020)

Add to Collection

Review Chemistry, Analytical

Electrochemical Sensing at a Confined Space

Si-Min Lu et al.

ANALYTICAL CHEMISTRY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Correlating Molecule Count and Release Kinetics with Vesicular Size Using Open Carbon Nanopipettes

Keke Hu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Add to Collection

Review Chemistry, Multidisciplinary

Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells

Ru-Jia Yu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

Add to Collection

Review Biochemistry & Molecular Biology

Mechanisms associated with biogenesis of exosomes in cancer

Kathleen M. McAndrews et al.

MOLECULAR CANCER (2019)

Add to Collection

Review Chemistry, Analytical

Confined Nanopipette-A new microfluidic approach for single cell analysis

Si-Min Lu et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2019)

Add to Collection

Article Nanoscience & Nanotechnology

Nanoscale tweezers for single-cell biopsies

Binoy Paulose Nadappuram et al.

NATURE NANOTECHNOLOGY (2019)

Add to Collection

Review Oncology

Unravelling tumour heterogeneity by single-cell profiling of circulating tumour cells

Laura Keller et al.

NATURE REVIEWS CANCER (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Chaotropic Monovalent Anion-Induced Rectification Inversion at Nanopipettes Modified by Polyimidazolium Brushes

Xiulan He et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Add to Collection

Article Chemistry, Analytical

Fabrication and Use of Nanopipettes in Chemical Analysis

Shudong Zhang et al.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 11 (2018)

Add to Collection

Article Chemistry, Multidisciplinary

Double Barrel Nanopores as a New Tool for Controlling Single-Molecule Transport

Paolo Cadinu et al.

NANO LETTERS (2018)

Add to Collection

Article Chemistry, Multidisciplinary

Electron-Transfer Gated Ion Transport in Carbon Nanopipets

Dengchao Wang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Add to Collection

Article Chemistry, Analytical

Direct Exosome Quantification via Bivalent-Cholesterol-Labeled DNA Anchor for Signal Amplification

Fang He et al.

ANALYTICAL CHEMISTRY (2017)

Add to Collection

Article Chemistry, Analytical

Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes

Yu-Min Wang et al.

ANALYTICAL CHEMISTRY (2017)

Add to Collection

Article Chemistry, Multidisciplinary

A Single-Cell Assay for Time Lapse Studies of Exosome Secretion and Cell Behaviors

Yu-Jui Chiu et al.

SMALL (2016)

Add to Collection

Article Chemistry, Analytical

Facile One-Step Photochemical Fabrication and Characterization of an Ultrathin Gold-Decorated Single Glass Nanopipette

Xiaolong Xu et al.

ANALYTICAL CHEMISTRY (2015)

Add to Collection

Article Cell Biology

Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles

Jinghuan Li et al.

JOURNAL OF EXTRACELLULAR VESICLES (2015)

Add to Collection

Article Cell Biology

Comparative marker analysis of extracellular vesicles in different human cancer types

Yusuke Yoshioka et al.

JOURNAL OF EXTRACELLULAR VESICLES (2013)

Add to Collection

Article Oncology

Hypoxic enhancement of exosome release by breast cancer cells

Hamish W. King et al.

BMC CANCER (2012)

Add to Collection

Article Nanoscience & Nanotechnology

Quantitative and qualitative flow cytometric analysis of nanosized cell-derived membrane vesicles

Esther N. M. Nolte-'t Hoen et al.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE (2012)

Add to Collection

Article Biochemistry & Molecular Biology

Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy

Huilin Shao et al.

NATURE MEDICINE (2012)

Add to Collection

Article Nanoscience & Nanotechnology

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

Rebecca A. Dragovic et al.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE (2011)

Add to Collection

Article Multidisciplinary Sciences

High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients

Mariantonia Logozzi et al.

PLOS ONE (2009)

Add to Collection

Article Multidisciplinary Sciences

Ceramide triggers budding of exosome vesicles into multivesicular Endosomes

Katarina Trajkovic et al.

SCIENCE (2008)

Add to Collection

© Peeref 2019-2024. All rights reserved.