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Summary: Rapid diagnosis of COVID-19 is crucial for controlling its spread, and electrochemical sensor platforms show great potential for detecting COVID-19 biomarkers. Point-of-care sensing allows for convenient detection and quantification of health biomarkers, and electrochemical biosensors play a significant role in this field. Integrating electrochemistry with COVID-19 diagnosis and screening using point-of-care technologies enables portable, user-friendly, and rapid analysis.
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Summary: CRISPR/Cas-based biosensors have been widely used for nucleic acid detection, but their applications for protein detection have been overlooked. Recent studies show that CRISPR/Cas-based biosensors not only analyze proteins, but also enhance the sensitivity and specificity of protein detection. However, there is a lack of comprehensive reviews and elucidation of the mechanisms of CRISPR/Cas-based biosensors for protein detection, which hinders the development of better protein biosensors.
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Summary: Integrated diagnostic devices are recognized as the ultimate solution to simplify healthcare testing, accelerating intervention and improving patient outcomes. The combination of non-invasive sampling and integrated devices is of substantial interest for improving patient comfort and safety. Factors affecting the transition of integrated devices from academia to market, such as technology readiness levels and survival rates, are crucial for success.
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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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Amanda Clifford et al.
Summary: Portable devices capable of rapid disease detection and health monitoring are crucial to decentralizing diagnostics from clinical laboratories to the patient point-of-need. New work in the field has targeted reagentless approaches to enable breakthroughs that will allow personalized monitoring of a wide range of biomarkers on demand. Reagentless platforms which allow for wash-free, calibration free-detection with increased dynamic range are highlighted as a key technological advance for autonomous sensing applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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Summary: A series of modified nucleoside triphosphates bearing dicarba-nido-undecaborate or metal bis(dicarbollide) groups were prepared and utilized for DNA synthesis and redox labeling. The combination of these redox labels allowed for the first fully orthogonal redox coding of all four canonical nucleobases, enabling determination of nucleobase composition of short DNA stretches with electrochemical readout in one simple primer extension experiment.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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Review
Biochemistry & Molecular Biology
Sam A. Spring et al.
Summary: Electrochemical biosensors offer accurate and sensitive analyte detection in opaque samples but are prone to signal drift and error. Inspired by fluorescence methods, scientists have developed ratiometric electrochemical protocols to improve sensor accuracy and reliability in recent years.
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Summary: This study presents a simple and sensitive electrochemical strategy for detecting telomerase extracted from HeLa cells, utilizing the signal amplification of in-situ formed streptavidin-biotin-DNA-biotin networks. The method effectively amplifies the signal by creating an insulating layer on a gold electrode surface, thus enabling the detection of telomerase.
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Summary: Electrochemical biosensors offer a promising approach for rapid on-the-spot sample analysis, but the main issue lies in developing platforms with high sensitivity and selectivity for analyzing low analyte concentrations in complex biological samples, due to nonspecific adsorption at the electrode interface.
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Brigitta R. Sun et al.
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Chemistry, Analytical
Si-Yuan Wang et al.
Summary: CRISPR/Cas proteins are powerful gene-editing tools and also show great promise in biosensing applications. Various nucleic acid-based signal amplification techniques can greatly improve detection sensitivity and specificity, extend the detectable target range, and enable diverse signal output modes in CRISPR/Cas-based biosensors.
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ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 14, 2021
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Review
Chemistry, Multidisciplinary
Andreas Frutiger et al.
Summary: The differentiation between specific and nonspecific binding in molecular interactions has been achieved in nature through precise control of biomolecules. However, artificial biosensors have struggled to overcome this issue. Through theoretical models and classification frameworks, it is possible to gain a better understanding and optimize the performance of biosensors, while exploring ways to address the challenge of nonspecific binding.
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Chemistry, Multidisciplinary
Richa Pandey et al.
Summary: This study presents an electrical assay that uses electroactive RNA-cleaving DNAzymes to identify specific bacterial targets and release a DNA barcode for rapid clinical analysis with high sensitivity and specificity. The assay demonstrates high selectivity for Escherichia coli and can accurately diagnose bacterial infections in a short amount of time.
Review
Engineering, Biomedical
Kaiyi Zhang et al.
Summary: The emergence and development of noninvasive biosensors have greatly facilitated the collection of physiological signals and health-related data. The utilization of machine learning algorithms enhances the accuracy and efficiency of biosensors, leading to significant advances in healthcare applications.
ADVANCED HEALTHCARE MATERIALS
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Materials Science, Biomaterials
Stefanie Fuchs et al.
Summary: Organ-on-chip systems are promising new tools for in vitro research in medical, pharmaceutical, and biological fields, offering improved resemblance to in vivo cell culture environments. Integration of in situ analysis methods on-chip allows for better time resolution and continuous measurements, with sensor technology playing a growing role in enhancing these systems.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Mengdi Bao et al.
Summary: CRISPR, with its unique properties, has emerged as a powerful molecular biosensing tool for nucleic acids and biomarkers. However, several key challenges need to be addressed for it to become a new gold standard. This paper reviews the history of biosensors, discusses current challenges and recent breakthroughs in CRISPR-based nucleic acid detection, and focuses on future advancements needed for CRISPR-based molecular biosensors.
Article
Engineering, Multidisciplinary
Ana Montero-Calle et al.
Summary: This study aimed to identify autoantigens specific to colorectal cancer (CRC) through liquid biopsy for early diagnosis. By analyzing the protein content, a total of 103 potential CRC-specific autoantigens were identified, and further validation showed dysregulation in protein levels of some, along with their association with CRC prognosis. Validation results demonstrated high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals, indicating the potential integration into a point-of-care device for CRC detection.
Review
Engineering, Biomedical
Xun Liu et al.
Summary: Nanomaterial-based electrochemical sensors possess unique properties and compatibility with other techniques, providing opportunities for biomedical applications. Integration with other techniques allows for precise health management and controlled drug release. Future research directions and challenges for commercial implementation of these sensors are presented.
ADVANCED NANOBIOMED RESEARCH
(2021)
Article
Chemistry, Analytical
Alejandro Valverde et al.
Summary: This study developed an electrochemical biosensing platform for detecting serum autoantibodies, utilizing specific peptides as receptors to achieve full diagnostic capability for Alzheimer's disease. The platform was further implemented in a POC multiplexing platform for efficient detection over clinically actionable times.
ANALYSIS & SENSING
(2021)
Article
Chemistry, Multidisciplinary
Ran Liu et al.
Summary: Home testing is an emerging strategy to combat the COVID-19 pandemic and prevent healthcare resource overload. Current diagnostic technologies for SARS-CoV-2 have drawbacks in terms of sensitivity, usability, and costs, making the tests unaffordable for most users at home. To address these limitations, a target-responsive CRISPR-PGM system has been proposed for translating SARS-CoV-2 detection into a glucose test, offering a potential single-device solution for monitoring multiple COVID-19 biomarkers at home.
Article
Chemistry, Multidisciplinary
Dongfei Chen et al.
Summary: An ultrasensitive electrochemical biosensor based on DNA-Au@MNPs has been developed for rapid detection of ctDNA in whole blood, with an ultralow detection limit and broad dynamic range. This sensor provides a promising method for achieving a 20-minute response time and minimally invasive early cancer diagnosis.
Article
Chemistry, Multidisciplinary
Meifang Wang et al.
Summary: The study demonstrates the rapid and sensitive electrochemical detection of doxorubicin using electrochemically pretreated glassy carbon electrodes modified with vertically-ordered mesoporous silica films. The sensor showed ultra-high sensitivity, low limit of detection, wide linear range, and the ability for direct and reliable detection of doxorubicin in human whole blood without complex sample pretreatments, thanks to the excellent anti-fouling and anti-interference properties of the VMSF.
Review
Chemistry, Multidisciplinary
Wei Feng et al.
Summary: The advancement of CRISPR-Cas technology has revolutionized genome editing and transcriptional modulation, as well as molecular detection of diverse targets. Integrating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, improved specificity and sensitivity, and development of point-of-care diagnostic techniques.
Article
Chemistry, Multidisciplinary
Nianzu Liu et al.
Summary: This study developed antifouling electrochemical biosensors based on designed all-in-one branched peptides that combine anchoring, doping, antifouling, and recognizing functions, enabling sensitive and reliable protein quantification in complex serum samples.
CHEMICAL COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Lingyin Meng et al.
BIOTECHNOLOGY ADVANCES
(2020)
Article
Chemistry, Analytical
Alejandro Valverde et al.
Article
Chemistry, Analytical
Lipei Luo et al.
Article
Chemistry, Analytical
Hanmei Deng et al.
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Luke A. Beardslee et al.
Article
Chemistry, Multidisciplinary
Juliane R. Sempionatto et al.
Review
Chemistry, Multidisciplinary
Feiyun Cui et al.
Article
Chemistry, Analytical
Yumeng Liao et al.
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Biophysics
Eva Vargas et al.
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Multidisciplinary
Netzahualcoyotl Arroyo-Curras et al.
Article
Medicine, Research & Experimental
Maria Garranzo-Asensio et al.
Article
Biochemical Research Methods
Kyungyeon Lee et al.
Review
Engineering, Biomedical
Il-Hoon Cho et al.
BIOMATERIALS RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Eva Vargas et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Biophysics
Lin Zhou et al.
BIOSENSORS & BIOELECTRONICS
(2019)
Review
Chemistry, Analytical
Nadeem Baig et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2019)
Review
Chemistry, Multidisciplinary
Can Dincer et al.
ADVANCED MATERIALS
(2019)
Article
Chemistry, Analytical
Jian Lu et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Yingying Tang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xia Li et al.
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Letter
Biotechnology & Applied Microbiology
Richard Bruch et al.
TRENDS IN BIOTECHNOLOGY
(2019)
Article
Chemistry, Analytical
Qian Wang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Biophysics
Magda A. Pereira-Barros et al.
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Multidisciplinary
Shuo Han et al.
Review
Chemistry, Multidisciplinary
Amanda Victorious et al.
FRONTIERS IN CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Beatriz L. Garrote et al.
Article
Chemistry, Analytical
Lele Wang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Yifan Dai et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Electrochemistry
Raquel Sanchez-Salcedo et al.
Article
Nanoscience & Nanotechnology
Jonathan Sabate del Rio et al.
NATURE NANOTECHNOLOGY
(2019)
Review
Chemistry, Analytical
Alba Iglesias-Mayor et al.
Review
Chemistry, Multidisciplinary
Yong Zhang et al.
Article
Biophysics
Shuopeng Liu et al.
BIOSENSORS & BIOELECTRONICS
(2018)
Article
Electrochemistry
Meijuan Liang et al.
Review
Chemistry, Medicinal
Oleksandra Pashchenko et al.
ACS INFECTIOUS DISEASES
(2018)
Article
Nanoscience & Nanotechnology
Roya Tavallaie et al.
NATURE NANOTECHNOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Victor Ruiz-Valdepenas Montiel et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Engineering, Biomedical
Jayoung Kim et al.
ADVANCED HEALTHCARE MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
Shana O. Kelley
Article
Biophysics
Wei Cai et al.
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Chemistry, Analytical
Victor Ruiz-Valdepenas Montiel et al.
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Prima Dewi Sinawang et al.
BIOSENSORS & BIOELECTRONICS
(2016)
Review
Chemistry, Analytical
Alfredo de la Escosura-Muniz et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2016)
Review
Chemistry, Multidisciplinary
Yongxi Zhao et al.
Review
Chemistry, Multidisciplinary
Darius G. Rackus et al.
CHEMICAL SOCIETY REVIEWS
(2015)
Article
Electrochemistry
Maria Bueno Serrano et al.
ELECTROCHEMISTRY COMMUNICATIONS
(2015)
Letter
Biochemical Research Methods
Lloyd M. Smith et al.
Review
Materials Science, Multidisciplinary
Hao Pei et al.
NPG ASIA MATERIALS
(2013)
Review
Chemistry, Multidisciplinary
Alfredo de la Escosura-Muniz et al.
Article
Biophysics
Fernando Javier Arevalo et al.
BIOSENSORS & BIOELECTRONICS
(2012)
Article
Biophysics
Susana Campuzano et al.
BIOSENSORS & BIOELECTRONICS
(2011)
Article
Chemistry, Multidisciplinary
Alfredo de la Escosura-Muniz et al.
Article
Chemistry, Analytical
Jie Wu et al.
ANALYTICAL CHEMISTRY
(2010)
Article
Electrochemistry
Alfredo de la Escosura-Muniz et al.
ELECTROCHEMISTRY COMMUNICATIONS
(2010)
