Journal
ROYAL SOCIETY OPEN SCIENCE
Volume 10, Issue 6, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rsos.230307
Keywords
annealing temperature; exons; Pentatomomorpha; touchdown hybridization; ultraconserved elements
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Target capture is commonly used in various fields of genetic research. The choice of hybridization temperature during the process has been investigated in vertebrates but not in invertebrates. Lower hybridization temperatures were found to enhance the recovery of targeted elements in leaf-footed bugs. Transcriptome-derived baits showed less sensitivity to hybridization temperature compared to baits developed from divergent genomes. Thus, using low hybridization temperatures can improve cost-effective invertebrate locus recovery.
Target capture is widely used in phylogenomic, ecological and functional genomic studies. Bait sets that allow capture from a diversity of species can be advantageous, but high-sequence divergence from baits can limit yields. Currently, only four experimental comparisons of a critical target capture parameter, hybridization temperature, have been published. These have been in vertebrates, where bait divergences are typically low, and none include invertebrates where bait-target divergences may be higher. Most invertebrate capture studies use a fixed, high hybridization temperature to maximize the proportion of on-target data, but many report low locus recovery. Using leaf-footed bugs (Hemiptera: Coreoidea), we investigate the effect of hybridization temperature on capture success of ultraconserved elements targeted by (i) baits developed from divergent hemipteran genomes and (ii) baits developed from less divergent coreoid transcriptomes. Lower temperatures generally resulted in more contigs and improved recovery of targets despite a lower proportion of on-target reads, lower read depth and more putative paralogues. Hybridization temperatures had less of an effect when using transcriptome-derived baits, which is probably due to lower bait-target divergences and greater bait tiling density. Thus, accommodating low hybridization temperatures during target capture can provide a cost-effective, widely applicable solution to improve invertebrate locus recovery.
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