Journal
FRONTIERS IN ENDOCRINOLOGY
Volume 14, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fendo.2023.1266985
Keywords
protein measurement; peptide quantification; steroid detection; LCMS (liquid chromatography-mass spectrometry); solid phase extraction
Categories
Ask authors/readers for more resources
The detection and quantification of hormones are crucial for assessing experimental models and diagnosing diseases. Traditional methods involve separate experiments, while the new approach allows simultaneous measurement of different categories of hormones. This study presents a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins.
The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available