Journal
ACS CENTRAL SCIENCE
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.3c00511
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GEN-Click is a genetically encoded copper-click reaction that allows spatial biotin labeling of azide or alkyne-tagged biomolecules in live cells. It provides a genetically controlled, spatially restricted, metal-catalyzed biorthogonal reaction for labeling various azido-bearing biomolecules.
GEN-Click is a genetically encoded copper-click reaction.It enables spatial biotin labeling of the azide or alkyne-tagged biomoleculesin live cells. Chemical reactions for the in situ modificationof biomolecules within living cells are under development. Among thesereactions, bio-orthogonal reactions such as click chemistry usingcopper(I) and Staudinger ligation are widely used for specific biomoleculetracking in live systems. However, currently available live cell copper(I)-catalyzedazide/alkyne cycloaddition reactions are not designed in a spatiallyresolved manner. Therefore, we developed the GEN-Clicksystem, which can target the copper(I)-catalyzed azide/alkyne cycloadditionreaction catalysts proximal to the protein of interest and can begenetically expressed in a live cell. The genetically controlled,spatially restricted, metal-catalyzed biorthogonal reaction can beused for proximity biotin labeling of various azido-bearing biomolecules(e.g., protein, phospholipid, oligosaccharides) in living cell systems.Using GEN-Click, we successfully detected local metabolite-transferringevents at cell-cell contact sites.
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