Journal
NATURAL PRODUCT RESEARCH
Volume 30, Issue 15, Pages 1731-1739Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2015.1137571
Keywords
Salix acutifolia; phenolic glycoside; benzoic acid glycoside; acutifoliside; UHPLC-MS; NMR
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Funding
- Biotechnology and Biological Sciences Research Council of the UK
- Cropping Carbon Institute Strategic Programme
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005199]
- Engineering and Physical Sciences Research Council [EP/K014676/1]
- BBSRC [BBS/E/C/00005199] Funding Source: UKRI
- EPSRC [EP/K014676/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005199] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K014676/1] Funding Source: researchfish
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Ultra high-performance liquid chromatography-mass spectrometry ( UHPLC-MS) profiling of a polar solvent extract of juvenile stem tissue of Salix acutifolia Willd. identified a range of phenolic metabolites. Salicortin, 1, a well-known salicinoid, was the major compound present and the study identified young stem tissue of this species as a potential source of this compound for future studies. Three further known metabolites ( salicin 2, catechin 3 and tremuloidin 4) were also present. The UHPLC-MS analysis also revealed the presence of a further, less polar, unknown compound, which was isolated via HPLC peak collection. The structure was elucidated by high-resolution mass spectroscopic analysis, 1- and 2-D NMR analysis and chemical derivatisation and was shown to be a novel benzoic acid glycoside 5, which we have named as acutifoliside. [GRAPHICS] .
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