Journal
ADVANCED SCIENCE
Volume -, Issue -, Pages -Publisher
WILEY
DOI: 10.1002/advs.202305096
Keywords
a cell-penetrant peptide; CP2c complex disruption; pan-anticancer drug; synthetic lethality; transcription factor CP2c
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This study presents a cell-penetrable peptide, ACP52C, that induces apoptosis in cancer cells through transcription activity-independent mechanisms. It dissociates transcription factor CP2c complexes and inhibits TDP2, leading to cancer-specific apoptosis. The study also finds that ACP52C can be sensitized by CASP2 inhibition and derivatives of ACP52C show improved pharmacokinetics and reduced cancer burden.
Despite advances in precision oncology, cancer remains a global public health issue. In this report, proof-of-principle evidence is presented that a cell-penetrable peptide (ACP52C) dissociates transcription factor CP2c complexes and induces apoptosis in most CP2c oncogene-addicted cancer cells through transcription activity-independent mechanisms. CP2cs dissociated from complexes directly interact with and degrade YY1, leading to apoptosis via the MDM2-p53 pathway. The liberated CP2cs also inhibit TDP2, causing intrinsic genome-wide DNA strand breaks and subsequent catastrophic DNA damage responses. These two mechanisms are independent of cancer driver mutations but are hindered by high MDM2 p60 expression. However, resistance to ACP52C mediated by MDM2 p60 can be sensitized by CASP2 inhibition. Additionally, derivatives of ACP52C conjugated with fatty acid alone or with a CASP2 inhibiting peptide show improved pharmacokinetics and reduced cancer burden, even in ACP52C-resistant cancers. This study enhances the understanding of ACP52C-induced cancer-specific apoptosis induction and supports the use of ACP52C in anticancer drug development.
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