4.8 Article

Bioorthogonal CRISPR/Cas9-Drug Conjugate: A Combinatorial Nanomedicine Platform

Related references

Note: Only part of the references are listed.
Review Pharmacology & Pharmacy

CRISPR/Cas9 ribonucleoprotein-mediated genome and epigenome editing in mammalian cells

Hanan Bloomer et al.

Summary: The CRISPR/Cas system has revolutionized the ability to edit the mammalian genome, providing a platform for correcting pathogenic mutations and studying gene function. Cas9 RNPs, as a delivery method, offer advantages in editing efficiency, off-target editing activity, and cellular toxicity reduction.

ADVANCED DRUG DELIVERY REVIEWS (2022)

Article Chemistry, Physical

Nano-assembly of a Chemically Tailored Cas9 Ribonucleoprotein for In Vivo Gene Editing and Cancer Immunotherapy

Juhee Lee et al.

Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.

CHEMISTRY OF MATERIALS (2022)

Article Biotechnology & Applied Microbiology

Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Jina Ko et al.

Summary: SAFE is a method for comprehensive longitudinal imaging of living cells using immunofluorescence, allowing multiparameter temporal and spatial imaging. It is non-toxic and functional in various living samples.

NATURE BIOTECHNOLOGY (2022)

Article Nanoscience & Nanotechnology

Enhancing CRISPR/Cas gene editing through modulating cellular mechanical properties for cancer therapy

Di Zhang et al.

Summary: Modulating the mechanical properties of tumor cells enhances gene editing and inhibits tumor growth by using dendrimer lipid nanoparticles for PD-L1 gene editing and knocking out FAK.

NATURE NANOTECHNOLOGY (2022)

Review Biotechnology & Applied Microbiology

Viral vector-based gene therapies in the clinic

Zongmin Zhao et al.

Summary: Gene therapies using viral vectors have shown promising outcomes in treating various diseases that were once considered incurable. The use of viral vectors allows for efficient delivery of gene therapeutics into target cells, leading to the approval of multiple products and ongoing clinical trials exploring their therapeutic potential.

BIOENGINEERING & TRANSLATIONAL MEDICINE (2022)

Review Pharmacology & Pharmacy

A Novel Anti-Cancer Therapy: CRISPR/Cas9 Gene Editing

Xin-Zhu Chen et al.

Summary: Cancer is a major cause of human deaths worldwide and places a heavy economic burden. Genetic and gene mutations are closely related to the occurrence, progression, and treatment prognosis of malignant tumors. The emergence of CRISPR/Cas9 offers hope for tumor treatment, but its off-target effects limit its clinical application.

FRONTIERS IN PHARMACOLOGY (2022)

Article Chemistry, Multidisciplinary

In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy

Jina Ko et al.

Summary: The ability to observe cells in live organisms is crucial for understanding their function in complex in vivo milieus. However, current methods have limited multiplexing capacity. In this study, a click chemistry-based strategy called scission-accelerated fluorophore exchange (SAFE) was developed for higher multiplexed in vivo imaging in mouse models. The method allows specific cell staining, complete un-staining, and non-toxic in vivo click chemistries at lower concentrations. It also enables cyclic imaging and has demonstrated potential utility in imaging immune cells with 12 colors.

ADVANCED SCIENCE (2022)

Editorial Material Oncology

Recent progress in antibody-drug conjugate therapy for cancer

Sara A. Hurvitz

Summary: ADCs, developed in the 1980s, have been approved by the US FDA and utilized in cancer treatment. In 2022, new ADCs were tested in clinical trials with promising outcomes.

NATURE CANCER (2022)

Article Chemistry, Multidisciplinary

Modularly Programmable Nanoparticle Vaccine Based on Polyethyleneimine for Personalized Cancer Immunotherapy

Jutaek Nam et al.

Summary: PEI-based nanoparticles show promise as a platform for personalized cancer vaccines, with the ability to activate immune cells and induce a strong CD8(+) T cell response against tumors.

ADVANCED SCIENCE (2021)

Article Chemistry, Multidisciplinary

Cas9 conjugate complex delivering donor DNA for efficient gene editing by homology-directed repair

Yoo Kyung Kang et al.

Summary: A Cas9 conjugate complex system was developed to induce efficient HDR editing with minimal carrier material. The Cas9-LP complexes showed lower cytotoxicity compared to conventional lipofectamine formulations and achieved efficient base correction of the RFP gene in HEK293T cells.

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Improving the efficiency of CRISPR-Cas12a-based genome editing with site-specific covalent Cas12a-crRNA conjugates

Xinyu Ling et al.

Summary: Researchers have improved the genome editing efficiency of the Cas12a system by covalently linking CRISPR RNA and Cas12a, achieving high-efficiency gene editing and providing a new approach for engineering other low-efficiency Cas family members.

MOLECULAR CELL (2021)

Review Medicine, Research & Experimental

CRISPR-cas9 genome editing delivery systems for targeted cancer therapy

Asma Ghaemi et al.

Summary: The prokaryotic CRISPR-Cas systems serve as a revolutionary genome editing tool for modifying, visualizing, and identifying DNA and RNA sequences in live cells of various species. Besides genome editing, the DNA-targeting modifications by CRISPR-Cas offer opportunities for diagnosis, therapy, and genetic disorders investigation. Preclinical studies and CRISPR-Cas9-based therapeutics may encounter challenges, but there is potential for its development in cancer therapy.

LIFE SCIENCES (2021)

Article Multidisciplinary Sciences

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva et al.

Summary: Efficiency and optimization of current CRISPR/Cas RNP delivery protocols to target cells will be assessed in this study using molecular cloning, protein expression and purification, cell culture, flow cytometry, and cellular imaging techniques. This will be the first extensive comparative study of popular CRISPR/Cas RNP delivery methods to human cell lines and primary cells.

PLOS ONE (2021)

Article Multidisciplinary Sciences

Bioorthogonal chemistry

Samuel L. Scinto et al.

Summary: Bioorthogonal chemistry involves high-yielding chemical reactions in biological environments that are rapid and selective, playing essential roles in various fields such as biomedical imaging and drug delivery. Its applications are diverse and include genetic code expansion, drug target identification, and antibody-drug conjugation.

NATURE REVIEWS METHODS PRIMERS (2021)

Article Chemistry, Multidisciplinary

Click activated protodrugs against cancer increase the therapeutic potential of chemotherapy through local capture and activation

Kui Wu et al.

Summary: The CAPAC platform introduces a novel approach to targeted cancer treatment by enabling the local activation of cytotoxic drugs specifically at tumor sites, overcoming systemic limitations and the need for specific biomarkers associated with traditional chemotherapy or targeted therapies.

CHEMICAL SCIENCE (2021)

Review Biotechnology & Applied Microbiology

Synthetic lethality as an engine for cancer drug target discovery

Alan Huang et al.

NATURE REVIEWS DRUG DISCOVERY (2020)

Article Biotechnology & Applied Microbiology

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

David N. Nguyen et al.

NATURE BIOTECHNOLOGY (2020)

Review Biochemistry & Molecular Biology

Recent advances of thiol-selective bioconjugation reactions

Philipp Ochtrop et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2020)

Review Medicine, Research & Experimental

Combinatorial drug therapy in cancer - New insights

Karanpreet Bhatia et al.

LIFE SCIENCES (2020)

Article Chemistry, Multidisciplinary

Rational design of minimum CRISPR guide RNA by site-specific Cas9-RNA conjugation

Xinyu Ling et al.

CHEMICAL COMMUNICATIONS (2020)

Review Chemistry, Multidisciplinary

Contemporary approaches to site-selective protein modification

Emily A. Hoyt et al.

NATURE REVIEWS CHEMISTRY (2019)

Article Nanoscience & Nanotechnology

A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing

Guojun Chen et al.

NATURE NANOTECHNOLOGY (2019)

Review Chemistry, Multidisciplinary

Multicomponent Reactions in Ligation and Bioconjugation Chemistry

Leslie Reguera et al.

ACCOUNTS OF CHEMICAL RESEARCH (2018)

Article Chemistry, Multidisciplinary

Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing

Romain Rouet et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Multidisciplinary Sciences

Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide

Hong-Xia Wang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Review Chemistry, Multidisciplinary

Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications

Chang Liu et al.

JOURNAL OF CONTROLLED RELEASE (2017)

Review Biotechnology & Applied Microbiology

Strategies and challenges for the next generation of antibody drug conjugates

Alain Beck et al.

NATURE REVIEWS DRUG DISCOVERY (2017)

Review Multidisciplinary Sciences

PARP inhibitors: Synthetic lethality in the clinic

Christopher J. Lord et al.

SCIENCE (2017)

Review Pharmacology & Pharmacy

Cell-Penetrating Peptides: From Basic Research to Clinics

Giulia Guidotti et al.

TRENDS IN PHARMACOLOGICAL SCIENCES (2017)

Article Multidisciplinary Sciences

Structural insights into DNA cleavage activation of CRISPR-Cas9 system

Cong Huai et al.

NATURE COMMUNICATIONS (2017)

Article Engineering, Biomedical

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

Kunwoo Lee et al.

NATURE BIOMEDICAL ENGINEERING (2017)

Article Chemistry, Multidisciplinary

Site-selective protein-modification chemistry for basic biology and drug development

Nikolaus Krall et al.

NATURE CHEMISTRY (2016)

Article Multidisciplinary Sciences

A chemical biology route to site-specific authentic protein modifications

Aerin Yang et al.

SCIENCE (2016)

Review Medicine, Research & Experimental

Synthetic Lethality and Cancer Therapy: Lessons Learned from the Development of PARP Inhibitors

Christopher J. Lord et al.

ANNUAL REVIEW OF MEDICINE, VOL 66 (2015)

Review Chemistry, Multidisciplinary

Advances in Chemical Protein Modification

Omar Boutureira et al.

CHEMICAL REVIEWS (2015)

Article Biochemistry & Molecular Biology

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Hiroshi Nishimasu et al.

Review Biochemistry & Molecular Biology

Development and Applications of CRISPR-Cas9 for Genome Engineering

Patrick D. Hsu et al.

Article Biochemistry & Molecular Biology

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

Suresh Ramakrishna et al.

GENOME RESEARCH (2014)

Review Medicine, General & Internal

Advances in using PARP inhibitors to treat cancer

Shivaani Kummar et al.

BMC MEDICINE (2012)

Review Biotechnology & Applied Microbiology

Combinatorial drug therapy for cancer in the post-genomic era

Bissan Al-Lazikani et al.

NATURE BIOTECHNOLOGY (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Review Chemistry, Multidisciplinary

Cu-free click cycloaddition reactions in chemical biology

John C. Jewett et al.

CHEMICAL SOCIETY REVIEWS (2010)

Article Chemistry, Multidisciplinary

Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli

JW Chin et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2002)