Journal
NANOTOXICOLOGY
Volume 10, Issue 6, Pages 736-748Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2015.1121412
Keywords
Liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS); omics data treatment; systems biology analysis; two-dimensional gel electrophoresis (2DE)
Categories
Funding
- European Commission Joint Research Center (JRC) within the Nanobiosciences (NBS) and Chemical Assessment and Testing (CAT) Units of the Institute for Health and Consumer Protection (IHCP) through the JRC Multiannual Work Program
Ask authors/readers for more resources
Omics technologies, such as proteomics or metabolomics, have to date been applied in the field of nanomaterial safety assessment to a limited extent. To address this dearth, we developed an integrated approach combining the two techniques to study the effects of two sizes, 5 and 30 nm, of gold nanoparticles (AuNPs) in Caco-2 cells. We observed differences in cells exposed for 72 h to each size of AuNPs: 61 responsive (up/down-regulated) proteins were identified and 35 metabolites in the cell extract were tentatively annotated. Several altered biological pathways were highlighted by integrating the obtained multi-omics data with bioinformatic tools. This provided a unique set of molecular information on the effects of nanomaterials at cellular level. This information was supported by complementary data obtained by immunochemistry, microscopic analysis, and multiplexed assays. A part from increasing our knowledge on how the cellular processes and pathways are affected by nanomaterials (NMs), these findings could be used to identify specific biomarkers of toxicity or to support the safe-by-design concept in the development of new nanomedicines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available