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Review of knockout technology approaches in bacterial drug resistance research

Journal

PEERJ
Volume 11, Issue -, Pages -

Publisher

PEERJ INC
DOI: 10.7717/peerj.15790

Keywords

Bacterial drug resistance; Gene knockout; Red homologous recombination; CRISPR; Cas9; Suicide plasmid

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Gene knockout is a widely used method in biology for investigating gene function. Red homologous recombination technology, CRISPR/Cas9 technology, and suicide plasmid vector systems have been the most extensively used for knocking out bacterial drug resistance genes. These technologies have shown significant results in researching bacterial gene functions. This study provides an overview of current gene knockout methods for genetic drug resistance testing in bacteria and aims to serve as a reference for selecting appropriate techniques.
Gene knockout is a widely used method in biology for investigating gene function. Several technologies are available for gene knockout, including zinc-finger nuclease technology (ZFN), suicide plasmid vector systems, transcription activator-like effector protein nuclease technology (TALEN), Red homologous recombination technology, CRISPR/Cas, and others. Of these, Red homologous recombination technology, CRISPR/Cas9 technology, and suicide plasmid vector systems have been the most extensively used for knocking out bacterial drug resistance genes. These three technologies have been shown to yield significant results in researching bacterial gene functions in numerous studies. This study provides an overview of current gene knockout methods that are effective for genetic drug resistance testing in bacteria. The study aims to serve as a reference for selecting appropriate techniques.

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