Protective effect of fustin against adjuvant-induced arthritis through the restoration of proinflammatory response and oxidative stress

Rheumatoid arthritis causes irreparable damage to joints. The present research sought to check fustin's anti-arthritic efficacy against the complete Freund's adjuvant-induced arthritis paradigm in animals by altering the inflammatory response. In the rats, complete Freund's adjuvant was used to trigger arthritis and they received fustin at 50 and 100 mg/kg for 21 days. At regular intervals, the hind paw volume and arthritic score were assessed. After the trial period, hematological, antioxidant, pro-inflammatory cytokines, and other biochemical parameters were estimated. Fustin-treated rats showed the down-regulation of hind paw volume, arthritic score, and altered hematological parameters (TLC, DLC (neutrophil, lymphocyte, monocyte, eosinophil, basophil)). Furthermore, fustin significantly mitigates proinflammatory cytokine (reduced interleukin, tumor necrosis factor-a (TNF-& alpha;), IL-6, IL-1 & beta;), oxidative stress (attenuated malondialdehyde (MDA), catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)), attenuated production of prostaglandin E2 and myeloperoxidase (MPO) and improved nuclear factor erythroid 2-related factor (Nrf2) action. Fustin led to the benefit in arthritis-prone animals elicited by complete Freund's adjuvant via proinflammatory cytokine.

Automated summary

The study aimed to evaluate the anti-arthritic effect of fustin on animals with complete Freund's adjuvant-induced arthritis by altering the inflammatory response. Rats were induced with arthritis using complete Freund's adjuvant and treated with fustin at doses of 50 and 100 mg/kg for 21 days. The results showed that fustin was able to reduce hind paw volume, arthritic score, and improve hematological parameters. Additionally, it exhibited anti-inflammatory, antioxidant, and immunomodulatory effects.