Journal
NANOSCALE RESEARCH LETTERS
Volume 11, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/s11671-016-1715-z
Keywords
Protein nanopore; alpha-Hemolysin; Gram-negative bacteria; Antimicrobial peptides; Bacteria biosensing
Funding
- National Research Foundation of Korea (NRF) [2016R1A2A1A05005440]
- Global Research Laboratory (GRL) [NRF-2014K1A1A2064460]
- [64/01.10.2015 PN-II-RU-TE-2014-4-2388]
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We present herein a first proof of concept demonstrating the potential of a protein nanopore-based technique for real-time detection of selected Gram-negative bacteria (Pseudomonas aeruginosa or Escherichia coli) at a concentration of 1.2 x 10(8) cfu/mL. The anionic charge on the bacterial outer membrane promotes the electrophoretically driven migration of bacteria towards a single alpha-hemolysin nanopore isolated in a lipid bilayer, clamped at a negative electric potential, and followed by capture at the nanopore's mouth, which we found to be described according to the classical Kramers' theory. By using a specific antimicrobial peptide as a putative molecular biorecognition element for the bacteria used herein, we suggest that the detection system can combine the natural sensitivity of the nanopore-based sensing techniques with selective biological recognition, in aqueous samples, and highlight the feasibility of the nanopore-based platform to provide portable, sensitive analysis and monitoring of bacterial pathogens.
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