Journal
NANOSCALE
Volume 8, Issue 2, Pages 1100-1107Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr07044a
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Funding
- National Natural Science Foundation of China [81361140345, 21505027, 51373043, 21535001]
- Chinese Academy of Sciences [XDA09030305]
- Ministry of Health [2012ZX10001-008]
- CAS/SAFEA International Partnership Program for Creative Research Teams
- Wang Bao-en Liver Fibrosis Foundation [2011029]
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This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.
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