4.7 Article

Performance of mNGS in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in non-neutropenic patients

Related references

Note: Only part of the references are listed.
Article Immunology

Performance of the Bronchoalveolar Lavage Fluid Aspergillus Galactomannan Lateral Flow Assay With Cube Reader for Diagnosis of Invasive Pulmonary Aspergillosis: A Multicenter Cohort Study

Jeffrey D. Jenks et al.

Summary: The study demonstrated that the Aspergillus Galactomannan Lateral Flow Assay showed good diagnostic performance for invasive aspergillosis, and its performance was consistent across different patient cohorts and centers.

CLINICAL INFECTIOUS DISEASES (2021)

Article Pathology

Viral metagenomic sequencing in the diagnosis of meningoencephalitis: a review of technical advances and diagnostic yield

Ellen C. Carbo et al.

Summary: Recent advances in metagenomics testing methodologies, both wet and dry lab, have provided additional etiological findings for meningoencephalitis patients. However, the diagnostic yield of metagenomic sequencing as a secondary test remains unknown. The current established infrastructure for bioinformatic analysis of SARS-CoV-2 may pave the way for global use of clinical metagenomics.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

Article Immunology

Metagenomic Next-Generation Sequencing for Pathogen Detection and Transcriptomic Analysis in Pediatric Central Nervous System Infections

Nanda Ramchandar et al.

Summary: Pediatric CNS infections are potentially life-threatening and traditional testing methods may not always identify the causative pathogen. This study showed that metagenomic sequencing of CSF has the potential to rapidly and accurately identify pathogens, providing a promising diagnostic tool for children with CNS infections.

OPEN FORUM INFECTIOUS DISEASES (2021)

Article Infectious Diseases

Metagenomic Next-Generation Sequencing for Pulmonary Fungal Infection Diagnosis: Lung Biopsy versus Bronchoalveolar Lavage Fluid

Lei Yang et al.

Summary: The study found that lung biopsy and BALF-based mNGS showed no difference in sensitivity and specificity in diagnosing pulmonary fungal infections, and outperformed conventional tests. The combination of lung biopsy and BALF for mNGS demonstrated higher diagnostic efficacy in comparison to BALF-mNGS alone.

INFECTION AND DRUG RESISTANCE (2021)

Article Medicine, General & Internal

Multicenter assessment of shotgun metagenomics for pathogen detection

Donglai Liu et al.

Summary: This study evaluated the performance of shotgun metagenomics in different laboratories, revealing that assay performance is influenced by various factors, with microbial type, host context, and read depth being important determinants. The study also suggested that effective pathogen detection using laboratory-developed shotgun metagenomics tests requires high-depth detection of specific microbes within a certain time frame.

EBIOMEDICINE (2021)

Article Medicine, General & Internal

Metagenomic next-generation sequencing to identify pathogens and cancer in lung biopsy tissue

Yifan Guo et al.

Summary: The study established a pipeline for simultaneous detection of pathogens and cancer using Illumina sequencing of lung biopsy tissue, achieving high sensitivity and specificity in infection and cancer diagnosis. The Nanopore platform showed lower sensitivity in pathogen detection compared to Illumina.

EBIOMEDICINE (2021)

Article Microbiology

Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis

Wang-Da Liu et al.

Summary: The study suggests that mNGS may serve as an adjunctive diagnostic tool for identifying pathogens in patients with hematologic malignancies presenting with sepsis.

MICROORGANISMS (2021)

Article Medicine, General & Internal

Comparing the diagnostic value of bronchoalveolar lavage fluid galactomannan, serum galactomannanan, and serum 1,3-β-D-glucan in non-neutropenic respiratory disease patients with invasive pulmonary aspergillosis

Zhang Dai et al.

Summary: This study evaluated the diagnostic performance of bronchoalveolar lavage fluid (BALF) galactomannan (GM) in non-neutropenic respiratory disease patients with invasive pulmonary aspergillosis (IPA), and found that BALF GM outperforms other biomarkers in diagnosis with an optimal cutoff value of 0.6.

MEDICINE (2021)

Article Biotechnology & Applied Microbiology

Diagnostic Accuracy of Metagenomic Next-Generation Sequencing in Sputum-Scarce or Smear-Negative Cases with Suspected Pulmonary Tuberculosis

Ning Zhu et al.

Summary: The study found that metagenomic next-generation sequencing (mNGS) showed higher sensitivity and specificity in bronchoalveolar lavage fluid (BALF) samples or lung tissue biopsy specimens from suspected pulmonary tuberculosis patients compared to traditional tests and T-SPOT.TB assay.

BIOMED RESEARCH INTERNATIONAL (2021)

Article Immunology

Metagenomic Next-Generation Sequencing for Pathogenic Diagnosis and Antibiotic Management of Severe Community-Acquired Pneumonia in Immunocompromised Adults

Ting Sun et al.

Summary: The study compared the pathogenic diagnosis performance of immunocompromised and immunocompetent patients with severe community-acquired pneumonia (SCAP) using metagenomic next-generation sequencing (mNGS). It was found that immunocompromised patients had a higher rate of mixed pathogens and required more antibiotic adjustments compared to immunocompetent patients. Metagenomic next-generation sequencing (mNGS) may be a useful technique for detecting mixed pathogens and guiding personalized antibiotic treatment in immunocompromised SCAP patients.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

Article Microbiology

Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA

Yacine Amar et al.

Summary: The Benzonase-based approach (BDA) was investigated in this study to pre-digest unprotected DNA of dead microbial cells, offering a more accurate assessment of the living microbiome and depletion of host DNA reads. The method resulted in less reads from dead bacteria and efficiently depleted host DNA reads in samples with high human-to-microbial DNA ratios.

MICROBIOME (2021)

Article Biochemistry & Molecular Biology

Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids

Wei Gu et al.

Summary: The developed mNGS test using cell-free DNA from body fluids demonstrates high sensitivity and specificity for identifying pathogens, showing potential clinical utility. This method shows promise in rapid pathogen detection and may accelerate clinical decisions by providing high-specificity, unbiased detection from diverse body fluids using metagenomic sequencing.

NATURE MEDICINE (2021)

Article Infectious Diseases

Application of metagenomic next-generation sequencing for bronchoalveolar lavage diagnostics in critically ill patients

Ying Li et al.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2020)

Review Medicine, General & Internal

Pulmonary Aspergillosis: What the Generalist Needs to Know

Firas El-Baba et al.

AMERICAN JOURNAL OF MEDICINE (2020)

Article Immunology

Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children

Matt S. Zinter et al.

CLINICAL INFECTIOUS DISEASES (2019)

Article Medicine, General & Internal

Clinical Metagenomic Sequencing for Diagnosis of Meningitis and Encephalitis

M. R. Wilson et al.

NEW ENGLAND JOURNAL OF MEDICINE (2019)

Review Infectious Diseases

Molecular Diagnostic Advances in Transplant Infectious Diseases

Brittany A. Young et al.

CURRENT INFECTIOUS DISEASE REPORTS (2019)

Review Respiratory System

Advances in the diagnosis and management of pulmonary aspergillosis

Yuqing Gao et al.

ADVANCES IN RESPIRATORY MEDICINE (2019)

Article Gastroenterology & Hepatology

Diagnosis of local hepatic tuberculosis through next-generation sequencing: Smarter, faster and better

Jing-Wen Ai et al.

CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY (2018)

Article Immunology

Detection of Pulmonary Infectious Pathogens From Lung Biopsy Tissues by Metagenomic Next-Generation Sequencing

Henan Li et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2018)

Article Pathology

Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection

Wei Gu et al.

Annual Review of Pathology-Mechanisms of Disease (2018)

Article Multidisciplinary Sciences

Integrating host response and unbiased microbe detection for lower respiratory tract infection diagnosis in critically ill adults

Charles Langelier et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Biology

Cutting Edge: Towards PubMed 2.0

Nicolas Fiorini et al.

ELIFE (2017)

Article Biotechnology & Applied Microbiology

The plasma virome of febrile adult Kenyans shows frequent parvovirus B19 infections and a novel arbovirus (Kadipiro virus)

Carolyne N. Ngoi et al.

JOURNAL OF GENERAL VIROLOGY (2016)

Review Respiratory System

Pulmonary aspergillosis: a clinical review

M. Kousha et al.

EUROPEAN RESPIRATORY REVIEW (2011)

Article Critical Care Medicine

Galactomannan in bronchoalveolar lavage fluid - A tool for diagnosing aspergillosis in intensive care unit patients

Wouter Meersseman et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2008)