Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 51, Issue 1, Pages 549-559Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2023.2268167
Keywords
Silver nanoparticles; Strobilanthes crispus; antibacterial; Streptococcus mutans; Escherichia coli; Pseudomonas aeruginosa
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This study synthesized stable and enhanced antibacterial Strobilanthes crispus-mediated silver nanoparticles (SC-AgNPs). Characterization and analysis revealed that SC-AgNPs exhibited significant antimicrobial activities against Escherichia coli, Pseudomonas aeruginosa, and Streptococcus mutans, and disrupted the gene expression of bacteria.
This study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis of AgNPs. The synthesized AgNPs were characterized by using UV-Vis spectrophotometer, dynamic light scattering (DLS), field emission scanning electron microscope (FESEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR). FESEM images showed a rough surface with a spherical shape. The average size distribution of 75.25 nm with a polydispersity index (PDI) of 0.373. XRD analysis matched the face-centred cubic structure of silver. FTIR analysis revealed a shifted peak from 1404.99 to 1345.00 cm-1. MIC and MBC values of SC-AgNPs were 1.25 mg/mL and 2.5 mg/mL against E. coli, P. aeruginosa and S. mutans, respectively. Time-kill assay showed that SC-AgNPs significantly reduced bacterial growth as compared to non-treated bacteria. Morphologies of bacteria treated with SC-AgNPs were shrunk, lysed, irregular and smaller as compared to control. SC-AgNPs significantly disrupted the gene expression of eae A, gtf B and Pel A (p < 0.05). This study indicated that the synthesized SC-AgNPs were stable with enhanced antibacterial activities.
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