Journal
NANOSCALE
Volume 8, Issue 1, Pages 309-317Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr06876b
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21431006, 91227103, 31370983]
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China [21521001]
- National Basic Research Program of China [2014CB931800, 2013CB933900]
- Scientific Research Grant of Hefei Science Center of Chinese Academy of Sciences [2015SRG-HSC038]
- Anhui Province Funds for Distinguished Young Scientists [1508085J08]
- Anhui Medical University
Ask authors/readers for more resources
Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation. Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach. Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable. Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration. According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process. By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained. Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products. Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available