Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 12, Issue 6, Pages 1499-1509Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2016.02.006
Keywords
Vibrio cholerae; ZnO nanoparticle; Antibacterial activity; Reactive oxygen species; Membrane fluidity; Biofilms
Funding
- Department of Science and Technology, India
- Council of Scientific and Industrial Research, India
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The potency of zinc oxide nanoparticles (NPs), with a core size of similar to 7-10 nm, to inhibit cholera disease was investigated by demonstrating the effect on two biotypes (classical and El Tor) of O1 serogroup of Vibrio cholerae-El Tor was more susceptible both in planktonic and in biofilm forms. Interaction with ZnO NP results in deformed cellular architecture. Increased fluidity and depolarization of membrane, and protein leakage further confirmed the damages inflicted on Vibrio by NP. NP was shown to produce reactive oxygen species (ROS) and induce DNA damage. These results suggest that the antibacterial mechanism of ZnO action is most likely due to generation of ROS and disruption of bacterial membrane. The antimicrobial efficacy of NP has been validated in animal model. The synergistic action of NP and antibiotic suggests an alternative for the treatment of cholera. (C) 2016 Elsevier Inc. All rights reserved.
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