Identifying candidate de novo genes expressed in the somatic female reproductive tract of Drosophila melanogaster

Most eukaryotic genes have been vertically transmitted to the present from distant ancestors. However, variable gene number across species indicates that gene gain and loss also occurs. While new genes typically originate as products of duplications and rearrangements of preexisting genes, putative de novo genes-genes born out of ancestrally nongenic sequence-have been identified. Previous studies of de novo genes in Drosophila have provided evidence that expression in male reproductive tissues is common. However, no studies have focused on female reproductive tissues. Here we begin addressing this gap in the literature by analyzing the transcriptomes of 3 female reproductive tract organs (spermatheca, seminal receptacle, and parovaria) in 3 species-our focal species, Drosophila melanogaster-and 2 closely related species, Drosophila simulans and Drosophila yakuba, with the goal of identifying putative D. melanogaster-specific de novo genes expressed in these tissues. We discovered several candidate genes, located in sequence annotated as intergenic. Consistent with the literature, these genes tend to be short, single exon, and lowly expressed. We also find evidence that some of these genes are expressed in other D. melanogaster tissues and both sexes. The relatively small number of intergenic candidate genes discovered here is similar to that observed in the accessory gland, but substantially fewer than that observed in the testis.

Automated summary

Most eukaryotic genes are vertically transmitted from ancestors, but gene gain and loss can also occur, resulting in variable gene numbers across species. Previous studies in Drosophila have shown that de novo genes, which are born from non-genic ancestral sequences, are commonly expressed in male reproductive tissues. However, there is a lack of research on female reproductive tissues. In this study, we analyzed the transcriptomes of three female reproductive tract organs in three Drosophila species to identify putative D. melanogaster-specific de novo genes.