Journal
NANOMEDICINE
Volume 11, Issue 14, Pages 1787-1800Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2016-0144
Keywords
apoptosis; corneal endothelium; gene therapy; magnetic nanoparticles; magnetofection
Funding
- German Research Foundation [FOR917, PL 281/3-1, INST 410/45-1, LSM 780]
- German Academic Exchange Service (DAAD)
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Aim: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs). Materials & methods: Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells. Results: Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs. Conclusion: Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.
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