Microwave-Assisted Syntheses of Rhodamine, Rhodol, and Fluorescein Derivatives

A series of rhodol-; fluorescein- and rhodamines-based spirolactam compounds, bearing electron donor amines have been prepared. For this purpose we have redesigned the synthesis of the rhodol scaffold using 2-(2,4-dihydroxybenzoyl)benzoic acid obtaining one example rhodol methyl ester in good yields (25-30 %) Thus, one set of non-cytotoxic rhodamine-based compounds has been prepared using thermal and microwave assisted synthesis (40-78 %) and tested as high affinity ATP chemo-sensors.

Automated summary

A series of spirolactam compounds based on rhodol, fluorescein, and rhodamines, which contain electron donor amines, were successfully synthesized. By redesigning the synthesis of the rhodol scaffold using 2-(2,4-dihydroxybenzoyl)benzoic acid, a rhodol methyl ester was obtained in good yields (25-30%). Non-cytotoxic rhodamine-based compounds were prepared using thermal and microwave-assisted synthesis (40-78%) and tested as high affinity ATP chemo-sensors.