☆ 4.7 Article

A Minispidroin Guides the Molecular Design for Cellular Condensation Mechanisms in S. cerevisiae

ACS SYNTHETIC BIOLOGY (2023)

Related references

Note: Only part of the references are listed.

Article Biochemistry & Molecular Biology

Programmable synthetic biomolecular condensates for cellular control

Yifan Dai et al.

Summary: The formation of biomolecular condensates through associative and segregative phase transitions is crucial for controlling cellular functions. This inspired the design of synthetic systems, but most research has focused on phase behaviors in a test tube. In this study, a rational engineering approach was used to program the formation and physical properties of synthetic condensates, achieving specific cellular functions such as plasmid sequestration and transcription regulation in bacteria, and protein circuit modulation in mammalian cells. This approach lays the foundation for engineering designer condensates for synthetic biology applications.

NATURE CHEMICAL BIOLOGY (2023)

Add to Collection

Review Chemistry, Multidisciplinary

Protein-Based Biological Materials: Molecular Design and Artificial Production

Ali Miserez et al.

Summary: Materials produced from natural biopolymers provide a sustainable alternative to synthetic polymers, with harmless degradation products. This review explores the chemical design and biofabrication of protein-based materials, including their primary sequences and structural levels, as well as their mechanical response. The latest research on artificially producing protein-based materials using biotechnology and synthetic biology is also discussed.

CHEMICAL REVIEWS (2023)

Add to Collection

Editorial Material Biochemistry & Molecular Biology

The dimerization mechanism of the N-terminal domain of spider silk proteins is conserved despite extensive sequence divergence

Medoune Sarr et al.

Summary: The N-terminal domain of spider silk proteins plays a crucial role in the storage and assembly of spider silk. In this study, the solution and crystal structures of the N-terminal domain of the flagelliform spidroin were determined. It was found that the electrostatic intermolecular interaction between specific residues is conserved, but the protonation events involve different residues compared to another type of spidroin. This suggests that the overall mechanism of pH-dependent dimerization is conserved but can be mediated by different pathways in different silk types.

JOURNAL OF BIOLOGICAL CHEMISTRY (2022)

Add to Collection

Review Biotechnology & Applied Microbiology

Recombinant Spider Silk: Promises and Bottlenecks

Maryam Ramezaniaghdam et al.

Summary: Spider silk has exceptional mechanical properties and potential applications. Scientists have attempted to produce spider silk proteins on a large scale through recombinant production in various host organisms, with a focus on plant systems.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

Add to Collection

Review Biochemistry & Molecular Biology

Molecular and environmental determinants of biomolecular condensate formation

Jose A. Villegas et al.

Summary: In this Review, the formation and regulation of biomolecular condensates are discussed. The physical principles and characteristics of phase-separating biomolecules are introduced, emphasizing the impact of molecular features on phase separation. The role of protein sequence properties in mediating intermolecular interactions is also explored. Additionally, the influence of the intracellular environment on protein structure and sequence determinants, as well as the modulation of physical parameters of phase transitions, is reviewed.

NATURE CHEMICAL BIOLOGY (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Principles Governing the Phase Separation of Multidomain Proteins

Priyesh Mohanty et al.

Summary: This article introduces the liquid-liquid phase separation mechanism of biological condensates and the role of multidomain proteins in phase separation. The article points out that the structure and interactions of multidomain proteins have important effects on the formation and function of condensates, and highlights the need for improvement in computational models.

BIOCHEMISTRY (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences

Mikael V. Garabedian et al.

Summary: Many proteins can undergo phase separation to form condensates, and engineered proteins with low complexity or intrinsically disordered sequences can form programmable synthetic organelles. Phase separation is affected by polypeptide interactions, and multivalency enhances phase separation. The authors developed a noncovalent strategy to multimerize a disordered protein and regulate condensate formation using protein interaction domains. They achieved temporal control over phase separation and optically induced condensation in cell-sized emulsions and live cells.

BIOCHEMISTRY (2022)

Add to Collection

Article Engineering, Biomedical

Recombinant protein condensation inside E. coli enables the development of building blocks for bioinspired materials engineering - Biomimetic spider silk protein as a case study

Bartosz Gabryelczyk et al.

Summary: It has been found that proteins can form liquid-like condensates during recombinant expression, which can transition to a solid-like state in response to changes in their microenvironment. This process could be used to select proteins for materials applications and structure-function studies.

MATERIALS TODAY BIO (2022)

Add to Collection

Article Multidisciplinary Sciences

Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein

Manjia Li et al.

Summary: This article introduces a method to control the activity of artificial synthetic condensates through light-triggered phase transition. These condensates, similar to membraneless organelles (MLOs), play important roles in biology. With this method, catalysis can be controlled within synthetic MLOs, and the phase transition behavior can be maintained in living cells.

NATURE COMMUNICATIONS (2022)

Add to Collection

Article Cell Biology

Identification of two pathways mediating protein targeting from ER to lipid droplets

Jiunn Song et al.

Summary: The study identifies two protein targeting pathways from the endoplasmic reticulum to lipid droplets and defines the key factors and mechanisms involved. These pathways play essential roles in the organization and function of eukaryotic cells.

NATURE CELL BIOLOGY (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Mussels Fabricate Porous Glues via Multiphase Liquid-Liquid Phase Separation of Condensates ARTICLE

Max Renner-Rao et al.

Summary: This study investigates the structure and formation process of mussel plaque, a bioinspired underwater glue composed of proteins, using 3D electron microscopic imaging. The study reveals that micro-and nanopores form spontaneously during the secretion of protein-filled secretory vesicles. Researchers also discovered that each vesicle contains a fluid condensate consisting of histidine and/or DOPA-rich proteins, which are likely the required ingredients for building the plaque. By rupturing vesicles under specific buffering conditions, controlled phase separation of different proteins occurs, resulting in the formation of a porous microplaque with fluid condensates within the pores. The results suggest that combining phase separation with tunable cross-linking kinetics could be effective for microfabricating hierarchically porous materials via self-assembly.

ACS NANO (2022)

Add to Collection

Article Chemistry, Physical

Biological colloids: Unique properties of membraneless organelles in the cell

Anna Bratek-Skicki et al.

Summary: Biomolecular condensates are membraneless organelles formed through liquid-liquid phase separation that can concentrate a variety of molecules in cells. They are highly dynamic and play crucial roles in cellular organization and physiology, with links to diseases like neurodegenerative disorders and cancer. This review discusses the mechanisms, dynamics, and evolution of these biological colloids, particularly focusing on their surface properties and interactions with other cellular components. Experimental approaches for characterizing the formation, interactions, and functions of these cellular colloidal organelles are also summarized.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Biological Materials Processing: Time-Tested Tricks for Sustainable Fiber Fabrication

Anna Rising et al.

Summary: This article explores how humans can learn from nature to sustainably fabricate polymeric fibers, by reviewing diverse model systems such as spider silk and mussel byssus. Common strategies are identified, highlighting the potential for bioinspired design and technology transfer.

CHEMICAL REVIEWS (2022)

Add to Collection

Article Biochemical Research Methods

A Reporter System for Cytosolic Protein Aggregates in Yeast

David Romero-Suarez et al.

Summary: Protein misfolding and aggregation are associated with neurodegenerative diseases and suboptimal protein expression in biotechnology. A promoter-reporter system based on yeast response to misfolded protein was developed to monitor protein aggregates, showing a 12-fold output dynamic range and the ability to distinguish different protein states. Optimization of promoters and culture conditions improved the performance of the reporter system.

ACS SYNTHETIC BIOLOGY (2021)

Add to Collection

Article Biochemistry & Molecular Biology

How do intrinsically disordered protein regions encode a driving force for liquid-liquid phase separation?

Wade Borcherds et al.

Summary: Liquid-liquid phase separation is crucial for the formation of biomolecular condensates, with disordered protein regions often driving this process. Understanding the molecular interactions and sequence properties of disordered protein regions is important, as dysfunction in phase separation can lead to diseases. A conceptual framework dividing IDRs into interacting and solvating regions has been useful in testing our understanding of experimental phase behavior.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Designer membraneless organelles sequester native factors for control of cell behavior

Mikael Garabedian et al.

Summary: The subcellular compartmentalization of macromolecules is essential for controlling biochemical reactions by increasing flux and preventing inhibitory interactions. We have developed a synthetic membraneless organelle platform that can control endogenous cellular activities by sequestering and insulating native proteins. By relocalizing targeted enzymes to synthetic condensates, we are able to efficiently regulate cellular behaviors such as proliferation, division, and cytoskeletal organization, and demonstrate strategies for releasing cargo from condensates to switch cells between functional states. These synthetic organelles offer a powerful and versatile approach to controlling cell decision-making in various model systems, with broad applications in cellular engineering.

NATURE CHEMICAL BIOLOGY (2021)

Add to Collection

Article Multidisciplinary Sciences

Microbial production of megadalton titin yields fibers with advantageous mechanical properties

Christopher H. Bowen et al.

Summary: The study engineered microbial production of high-performance fibers with properties similar to natural titin, but with higher strength, toughness, and damping energy than many synthetic and natural polymers. The unique inter-chain crystallization of folded immunoglobulin-like domains is suggested to be the key to these superior properties, allowing resistance to interchain slippage while permitting intra-chain unfolding. These fibers have potential applications in fields ranging from biomedicine to textiles, and the developed approach is expected to spur further innovation in microbial production of high-performance materials.

NATURE COMMUNICATIONS (2021)

Add to Collection

Review Cell Biology

A framework for understanding the functions of biomolecular condensates across scales

Andrew S. Lyon et al.

Summary: Biomolecular condensates are membraneless molecular assemblies formed via liquid-liquid phase separation, playing important roles in controlling biochemical reactions and regulating cell organization and function throughout eukaryotic cells.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2021)

Add to Collection

Article Biochemical Research Methods

SPLIT: Stable Protein Coacervation Using a Light Induced Transition

Ellen H. Reed et al.

ACS SYNTHETIC BIOLOGY (2020)

Add to Collection

Review Biochemistry & Molecular Biology

Making the Case for Disordered Proteins and Biomolecular Condensates in Bacteria

Megan C. Cohan et al.

TRENDS IN BIOCHEMICAL SCIENCES (2020)

Add to Collection

Article Cell Biology

Reorganization of budding yeast cytoplasm upon energy depletion

Guendalina Marini et al.

MOLECULAR BIOLOGY OF THE CELL (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Formation and functionalization of membraneless compartments inEscherichia coli

Shao-Peng Wei et al.

NATURE CHEMICAL BIOLOGY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

De novo engineering of intracellular condensates using artificial disordered proteins

Michael Dzuricky et al.

NATURE CHEMISTRY (2020)

Add to Collection

Review Biochemistry & Molecular Biology

Liquid-Liquid Phase Separation in Crowded Environments

Alain A. M. Andre et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Add to Collection

Article Materials Science, Multidisciplinary

Liquid-liquid phase separation of proteins and peptides derived from biological materials: Discovery, protein engineering, and emerging applications

Yue Sun et al.

MRS BULLETIN (2020)

Add to Collection

Article Multidisciplinary Sciences

Spider silk self-assembly via modular liquid-liquid phase separation and nanofibrillation

Ali D. Malay et al.

SCIENCE ADVANCES (2020)

Add to Collection

Review Biochemistry & Molecular Biology

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

Simon Alberti et al.

CELL (2019)

Add to Collection

Article Biophysics

Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching

Nicole O. Taylor et al.

BIOPHYSICAL JOURNAL (2019)

Add to Collection

Article Cell Biology

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

David T. McSwiggen et al.

GENES & DEVELOPMENT (2019)

Add to Collection

Article Polymer Science

Molecular crowding facilitates assembly of spidroin-like proteins through phase separation

Laura Lemetti et al.

EUROPEAN POLYMER JOURNAL (2019)

Add to Collection

Review Biotechnology & Applied Microbiology

Probing and engineering liquid-phase organelles

Dan Bracha et al.

NATURE BIOTECHNOLOGY (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Ion effects on the conformation and dynamics of repetitive domains of a spider silk protein: implications for solubility and β-sheet formation

Nur Alia Oktaviani et al.

CHEMICAL COMMUNICATIONS (2019)

Add to Collection

Article Biochemistry & Molecular Biology

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

Jie Wang et al.

CELL (2018)

Add to Collection

Review Biochemistry & Molecular Biology

Role of Liquid-Liquid Phase Separation in Assembly of Elastin and Other Extracellular Matrix Proteins

Lisa D. Muiznieks et al.

JOURNAL OF MOLECULAR BIOLOGY (2018)

Add to Collection

Article Multidisciplinary Sciences

Phase separation of a yeast prion protein promotes cellular fitness

Titus M. Franzmann et al.

SCIENCE (2018)

Add to Collection

Article Cell Biology

Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery

Sonja Kroschwald et al.

CELL REPORTS (2018)

Add to Collection

Article Materials Science, Multidisciplinary

Uncovering the structure–function relationship in spider silk

Jeffery L. Yarger et al.

Nature Reviews Materials (2018)

Add to Collection

Article Multidisciplinary Sciences

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

Benjamin S. Schuster et al.

NATURE COMMUNICATIONS (2018)

Add to Collection

Article Biology

Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers

Pezhman Mohammadi et al.

COMMUNICATIONS BIOLOGY (2018)

Add to Collection

News Item Engineering, Chemical

Decorated Spider Silk Slowly Releases Antibiotics

D. Harvey

CHEMICAL ENGINEERING PROGRESS (2017)

Add to Collection

Review Cell Biology

Biomolecular condensates: organizers of cellular biochemistry

Salman F. Banani et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2017)

Add to Collection

Review Multidisciplinary Sciences

Liquid phase condensation in cell physiology and disease

Yongdae Shin et al.

SCIENCE (2017)

Add to Collection

Article Chemistry, Physical

Self-coacervation of modular squid beak proteins - a comparative study

Hao Cai et al.

SOFT MATTER (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Stress-Triggered Phase Separation Is an Adaptive, Evolutionarily Tuned Response

Joshua A. Riback et al.

CELL (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Biomimetic spinning of artificial spider silk from a chimeric minispidroin

Marlene Andersson et al.

NATURE CHEMICAL BIOLOGY (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets

Yongdae Shin et al.

CELL (2017)

Add to Collection

Article Biology

A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

Matthias Christoph Munder et al.

ELIFE (2016)

Add to Collection

Article Biochemistry & Molecular Biology

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

Avinash Patel et al.

CELL (2015)

Add to Collection

Article Biochemistry & Molecular Biology

Toward spinning artificial spider silk

Anna Rising et al.

NATURE CHEMICAL BIOLOGY (2015)

Add to Collection

Article Biochemistry & Molecular Biology

Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient

YerPeng Tan et al.

NATURE CHEMICAL BIOLOGY (2015)

Add to Collection

Article Physics, Multidisciplinary

Polymer physics of intracellular phase transitions

Clifford P. Brangwynne et al.

NATURE PHYSICS (2015)

Add to Collection

Article Multidisciplinary Sciences

High-performance mussel-inspired adhesives of reduced complexity

B. Kollbe Ahn et al.

NATURE COMMUNICATIONS (2015)

Add to Collection

Article Engineering, Biomedical

A mussel-derived one component adhesive coacervate

Wei Wei et al.

ACTA BIOMATERIALIA (2014)

Add to Collection

Review Cell Biology

Liquid-Liquid Phase Separation in Biology

Anthony A. Hyman et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 30 (2014)

Add to Collection

Article Cell Biology

How do bacteria localize proteins to the cell pole?

Geraldine Laloux et al.

JOURNAL OF CELL SCIENCE (2014)

Add to Collection

Article Nanoscience & Nanotechnology

Strong underwater adhesives made by self-assembling multi-protein nanofibres

Chao Zhong et al.

NATURE NANOTECHNOLOGY (2014)

Add to Collection

Article Multidisciplinary Sciences

Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation

Nina Kronqvist et al.

NATURE COMMUNICATIONS (2014)

Add to Collection

Article Biotechnology & Applied Microbiology

Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science

Paul A. Guerette et al.

NATURE BIOTECHNOLOGY (2013)

Add to Collection

Article Multidisciplinary Sciences

Self-assembly of spider silk proteins is controlled by a pH-sensitive relay

Glareh Askarieh et al.

NATURE (2010)

Add to Collection

Article Biotechnology & Applied Microbiology

Expression of functional recombinant mussel adhesive protein Mgfp-5 in Escherichia coli

DS Hwang et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2004)

Add to Collection

Article Multidisciplinary Sciences

Extreme diversity, conservation, and convergence of spider silk fibroin sequences

J Gatesy et al.

SCIENCE (2001)

Add to Collection

© Peeref 2019-2024. All rights reserved.