Journal
NANO LETTERS
Volume 16, Issue 3, Pages 1746-1753Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b04676
Keywords
RNA nanotechnology; RNA nanoparticles; RNA interference; RNA-DNA hybrid reassociation
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Funding
- Frederick National Laboratory for Cancer Research, National Institutes of Health [HHSN261200800001E]
- UNC Charlotte
- Intramural Research Program of National Institutes of Health, Center for Cancer Research
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Current work reports the use of single-stranded RNA toeholds of different lengths to promote the reassociation of various RNA-DNA hybrids, which results in activation of multiple split functionalities inside human cells. The process of reassociation is analyzed and followed with a novel computational multistrand secondary structure prediction algorithm and various experiments. All of our previously designed RNA/DNA nanoparticles employed single stranded DNA toeholds to initiate reassociation. The use of RNA toeholds is advantageous because of the simpler design rules, the shorter toeholds, and the smaller size of the resulting nanoparticles (by up to 120 nucleotides per particle) compared to the same hybrid nanoparticles with single-stranded DNA toeholds. Moreover, the cotranscriptional assemblies result in higher yields for hybrid nanopartides with ssRNA toeholds.
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