Journal
NUTRIENTS
Volume 15, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/nu15214689
Keywords
fatty acid oxidation disorder; glycerol; glucose homeostasis; heptanoate; stable isotope; very long-chain acyl-CoA dehydrogenase; VLCAD deficiency
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Defects in mitochondrial fatty acid beta-oxidation impair energy homeostasis. Very-long-chain acyl-CoA dehydrogenase deficiency is the most common mitochondrial FAO disorder, causing hypoglycemia. Triheptanoin, a dietary treatment, can be metabolized into precursors for gluconeogenesis. Studying the role of gluconeogenesis in long-chain mitochondrial FAO defects, it was found that heptanoate effectively increased glucose production.
Defects in mitochondrial fatty acid beta-oxidation (FAO) impair metabolic flexibility, which is an essential process for energy homeostasis. Very-long-chain acyl-CoA dehydrogenase (VLCADD; OMIM 609575) deficiency is the most common long-chain mitochondrial FAO disorder presenting with hypoglycemia as a common clinical manifestation. To prevent hypoglycemia, triheptanoin-a triglyceride composed of three heptanoates (C7) esterified with a glycerol backbone-can be used as a dietary treatment, since it is metabolized into precursors for gluconeogenesis. However, studies investigating the effect of triheptanoin on glucose homeostasis are limited. To understand the role of gluconeogenesis in the pathophysiology of long-chain mitochondrial FAO defects, we injected VLCAD-deficient (VLCAD-/-) mice with 13C3-glycerol in the presence and absence of heptanoate (C7). The incorporation of 13C3-glycerol into blood glucose was higher in VLCAD-/- mice than in WT mice, whereas the difference disappeared in the presence of C7. The result correlates with 13C enrichment of liver metabolites in VLCAD-/- mice. In contrast, the C7 bolus significantly decreased the 13C enrichment. These data suggest that the increased contribution of gluconeogenesis to the overall glucose production in VLCAD-/- mice increases the need for gluconeogenesis substrate, thereby avoiding hypoglycemia. Heptanoate is a suitable substrate to induce glucose production in mitochondrial FAO defect.
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