A Comprehensive Assessment of the Biocompatibility and Safety of Diamond Nanoparticles on Reconstructed Human Epidermis

Diamond nanoparticles, also known as nanodiamonds (NDs), exhibit remarkable, aweinspiring properties that make them suitable for various applications in the field of skin care products. However, a comprehensive assessment of their compatibility with human skin, according to the irritation criteria established by the Organization for Economic Cooperation and Development (OECD), has not yet been conducted. The purpose of this study was to evaluate if diamond nanoparticles at a concentration of 25 mu g/mL, incubated with reconstituted human epidermis (EpiDerm(TM)) for 18 h, conform to the OECD TG439 standard used to classify chemical irritants. For this purpose, a cell viability test (MTT assay), histological assessment, and analysis of pro-inflammatory cytokine expression were performed. The results indicated that NDs had no toxic effect at the tested concentration. They also did not adversely affect tissue structure and did not lead to a simultaneous increase in protein and mRNA expression of the analyzed cytokines. These results confirm the safety and biocompatibility of NDs for application in skincare products, thereby creating a wide range of possibilities to exert an impact on the advancement of contemporary cosmetology in the future.

Automated summary

This study evaluated the compatibility of diamond nanoparticles with human skin according to OECD criteria. The results showed that diamond nanoparticles at a concentration of 25 μg/mL did not cause toxic effects or adverse effects on tissue structure and cytokine expression. These findings confirm the safety and biocompatibility of diamond nanoparticles for use in skincare products.