Journal
MATERIALS
Volume 16, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/ma16134641
Keywords
atherosclerosis; polysulfone; low-density lipoprotein; enzyme-linked immunosorbent assay
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In this study, polysulfone (PSF), sulfonated polysulfone (SPSF), and sulfonated polysulfone/dextran (SPSF/GLU) membranes were successfully fabricated for LDL adsorption. The SPSF/GLU membrane exhibited high resistance to protein adsorption and a similar adsorption capacity to PSF. The SPSF membrane showed excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in LDL removal.
The abnormal increase in low-density lipoprotein (LDL) in human blood is a main independent risk factor for the pathogenesis of atherosclerosis, whereas a reduced LDL level effectively lowers morbidity. It is important to develop LDL adsorption materials with high efficiency and selectivity, as well as to simplify their fabrication processes. In this paper, polysulfone (PSF), sulfonated polysulfone (SPSF), and sulfonated polysulfone/dextran (SPSF/GLU) membranes were successfully fabricated for LDL adsorption using a solution casting technique. Attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy measurements confirmed the success of the preparation. The water contact angle decreased from 89.7 & PLUSMN; 3.4 & DEG; (PSF) to 76.4 & PLUSMN; 3.2 & DEG; (SPSF) and to 71.2 & PLUSMN; 1.9 & DEG; (SPSF/GLU), respectively. BSA adsorption testing showed that the SPSF/GLU with surface enrichment of sulfonate groups and glycosyl groups possessed higher resistance to protein solution. The adsorption and desorption behaviors of the studied samples in single-protein or binary-protein solutions were systematically investigated by enzyme-linked immunosorbent assay (ELISA), The results showed that SPSF/GLU, which had excellent resistance to protein adsorption, possessed a similar adsorption capacity to that of PSF. SPSF membrane exhibited excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in LDL removal.
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