Multi-omics reveal the metabolic patterns in mouse cumulus cells during oocyte maturation

Bi-directional communication between cumulus cells and the surrounded oocytes is important for the development and functions of both compartments. However, the metabolic framework in cumulus cells has not been systematically described. In the present study, cumulus cells from cumulus-oocyte complexes (COCs) at three key time points were isolated (arrested GV stage, post-hCG 0h; meiotic resumption GVBD stage, post-hCG 3h; and metaphase II stage, post-hCG 12h), and the temporal metabolomic and proteomic profiling were performed. Integrated multi-omics analysis reveals the global metabolic patterns in cumulus cells during mouse oocyte maturation. In particular, we found the active hyaluronic acid metabolism, steroid hormone synthesis, and prostaglandin E2 (PGE2) production in cumulus cells. Meanwhile, accompanying the oocyte maturation, a progressive increase in nucleotide and amino acid metabolism was detected in the surrounding cumulus cells. In sum, the data serve as a valuable resource for probing metabolism during terminal differentiation of ovarian granulosa cells, and provide the potential biomarkers for improving and predicting oocyte quality.

Automated summary

In this study, the metabolic and proteomic profiling of cumulus cells during mouse oocyte maturation were analyzed. The findings revealed the active hyaluronic acid metabolism, steroid hormone synthesis, and prostaglandin E2 production in cumulus cells. Additionally, an increase in nucleotide and amino acid metabolism was observed in the surrounding cumulus cells as oocyte maturation progresses. These results provide valuable insights into the terminal differentiation of ovarian granulosa cells and have potential implications for improving and predicting oocyte quality.