The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genes

Combining genome assembly with population and functional genomics can provide valuable insights to development and evolution, as well as tools for species management. Here, we present a chromosome-level genome assembly of the common brushtail possum (Trichosurus vulpecula), a model marsupial threatened in parts of their native range in Australia, but also a major introduced pest in New Zealand. Functional genomics reveals post-natal activation of chemosensory and metabolic genes, reflecting unique adaptations to altricial birth and delayed weaning, a hallmark of marsupial development. Nuclear and mitochondrial analyses trace New Zealand possums to distinct Australian subspecies, which have subsequently hybridised. This admixture allowed phasing of parental alleles genome-wide, ultimately revealing at least four genes with imprinted, parent-specific expression not yet detected in other species (MLH1, EPM2AIP1, UBP1 and GPX7). We find that reprogramming of possum germline imprints, and the wider epigenome, is similar to eutherian mammals except onset occurs after birth. Together, this work is useful for genetic-based control and conservation of possums, and contributes to understanding of the evolution of novel mammalian epigenetic traits.

Automated summary

This study presents a chromosome-level genome assembly of the common brushtail possum and reveals insights into its development, evolution, and unique adaptations. Functional genomics uncover the activation of chemosensory and metabolic genes in post-natal stages, reflecting marsupial-specific traits. The analysis also reveals distinct Australian subspecies and hybridization events in New Zealand possums, providing valuable information for species control and conservation. The study sheds light on the epigenetic traits of novel mammalian species, contributing to the understanding of their evolution.