Genotoxicity of a thiosulfonate compound derived from Allium sp intended to be used in active food packaging: In vivo comet assay and micronucleus test

Components of Allium species have antimicrobial and antioxidant properties. A commercial Allium sp. extract (Proallium AP (R)), of which the main constituent is propyl thiosuiphinate oxide (PTSO), is being used in the development of active food packaging. In previous in vitro genotoxicity studies, PTSO, in the presence of metabolic activation, increased the appearance of micronuclei (MN). We assessed the genotoxicity PTSO in rats following oral administration (doses: 5.5, 17.4, and 55 mg/kg). The comet assay in liver and stomach (OECD 489) and the MN assay in bone marrow (OECD 474) were carried out. After necropsy, histopathological examinations of the liver and the stomach were performed. The results revealed no in vivo genotoxicity and the histopathological analysis showed only slight modifications, such as increased glycogen storage in the liver and a degenerative process in stomach, with vacuolization of cell membranes, only at the highest dose. Therefore, the present work confirms that this compound is not genotoxic and could be considered as a natural alternative to synthetic preservatives used in the food packaging industry. (C) 2016 Elsevier B.V. All rights reserved.