4.6 Article

Applications of the Microscale Thermophoresis Binding Assay in COVID-19 Research

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Structure of a volume-regulated heteromeric LRRC8A/C channel

Sonja Rutz et al.

Summary: Volume-regulated anion channels (VRACs) play a role in cellular response to osmotic swelling. These membrane proteins are composed of heteromeric assemblies of LRRC8 subunits, with compositions affecting permeation properties. In this study, the structure of murine LRRC8A/C channels was characterized. The assembly showed a predominant organization with an A:C ratio of two, with LRRC8A subunits stabilizing a closed state of the channel while LRRC8C subunits enhancing the activation properties.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2023)

Article Chemistry, Medicinal

In Silico and In Vitro Identification of Pan-Coronaviral Main Protease Inhibitors from a Large Natural Product Library

Nasim Shahhamzehei et al.

Summary: This study explored potential natural pan-coronavirus drugs using in vitro and in silico approaches, identifying hypericin, rosmarinic acid, isorhamnetin, and luteolin as the most efficient inhibitors of SARS-CoV-2 M-pro in vitro. These compounds also showed inhibitory effects on M-pro of other human-pathogenic coronaviruses, indicating potential as broad-spectrum anti-coronaviral agents.

PHARMACEUTICALS (2022)

Review Biochemistry & Molecular Biology

Microscale Thermophoresis as a Tool to Study Protein Interactions and Their Implication in Human Diseases

Romain Magnez et al.

Summary: This review highlights the crucial roles of protein-protein interactions (PPIs) in life processes and their involvement in various human diseases. It introduces Microscale Thermophoresis (MST) as a rapid, robust, and efficient alternative method for detecting therapeutically pertinent proteins. The review summarizes selected case studies and discusses the benefits and limitations of MST in studying PPIs and identifying regulators.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Letter Cell Biology

Structures of ACE2-SIT1 recognized by Omicron variants of SARS-CoV-2

Yaping Shen et al.

CELL DISCOVERY (2022)

Review Chemistry, Analytical

Microscale thermophoresis as a powerful growing analytical technique for the investigation of biomolecular interaction and the determination of binding parameters

Sami El Deeb et al.

Summary: This review explores the usefulness of microscale thermophoresis in studying molecular and biomolecular affinity interactions, and provides an overview of its current state and recent developments in the field. It also describes the principle of microscale thermophoresis and discusses its advantages, drawbacks, and application in different fields.

METHODS AND APPLICATIONS IN FLUORESCENCE (2022)

Article Multidisciplinary Sciences

Genetic variants determine intrafamilial variability of SARS-CoV-2 clinical outcomes in 19 Italian families

Alessia Azzara et al.

Summary: The clinical expression of COVID-19 is significantly influenced by the host genetic profile, showing high heterogeneity in symptoms and susceptibility to the disease. Familial analysis and global statistical analysis reveal significant associations between specific gene variants and SARS-CoV-2 infection and severe phenotypes.

PLOS ONE (2022)

Review Cell Biology

Aging in COVID-19: Vulnerability, immunity and intervention

Yiyin Chen et al.

Summary: The COVID-19 pandemic originating from Wuhan in December 2019 has had far-reaching health and socioeconomic impacts worldwide. The disease caused by SARS-CoV-2 is highly heterogeneous, with age, gender, and comorbidities playing key roles in disease severity and outcomes. Aging and dysregulation of immune function are thought to contribute to the increased vulnerability of older adults to severe COVID-19. Further research on immune responses to SARS-CoV-2 infection, particularly in different age groups, is crucial for understanding disease heterogeneity and developing effective vaccines.

AGEING RESEARCH REVIEWS (2021)

Review Biochemistry & Molecular Biology

COVID-19: A Global Challenge with Old History, Epidemiology and Progress So Far

Mujeeb Khan et al.

Summary: The article summarizes the three deadly pandemics associated with novel coronaviruses that humans have witnessed in the twenty-first century: SARS, MERS, and COVID-19. Specifically, it describes the epidemiological characteristics of COVID-19, such as transmission, death rate, and discusses various treatment strategies and different approaches to vaccine development.

MOLECULES (2021)

Review Cell Biology

COVID-19: Characteristics and Therapeutics

Rameswari Chilamakuri et al.

Summary: The novel coronavirus (COVID-19) has significantly impacted the world since its emergence at the end of 2019, but there have been major advancements in our understanding of the virus and development of treatment options and vaccines to control the pandemic.

CELLS (2021)

Article Biochemical Research Methods

The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters

Yurie Chatzikyriakidou et al.

Summary: Solute carrier (SLC) transporters, representing the second-largest fraction of the membrane proteome, have been underutilized as drug targets. The GFP thermal shift (GFP-TS) assay is a useful tool for detecting specific ligand interactions and affinities of SLC transporter fusions.

NATURE PROTOCOLS (2021)

Review Virology

COVID-19: A Review on the Novel Coronavirus Disease Evolution, Transmission, Detection, Control and Prevention

Anshika Sharma et al.

Summary: Coronaviruses, including the recent SARS-CoV-2, have caused major outbreaks since 2002, with the current COVID-19 pandemic infecting millions worldwide and posing a serious global health threat. The virus is constantly evolving and spreading through asymptomatic carriers, increasing the risk of transmission and highlighting the urgent need for effective control and prevention strategies.

VIRUSES-BASEL (2021)

Article Biotechnology & Applied Microbiology

Integrative approach identifies SLC6A20 and CXCR6 as putative causal genes for the COVID-19 GWAS signal in the 3p21.31 locus

Silva Kasela et al.

Summary: The study identifies SLC6A20 and CXCR6 as potential causal genes that modulate COVID-19 risk by integrating genome-scale CRISPR loss-of-function screens and eQTLs in diverse cell types and tissues. This integrative approach bridges the gap between correlational and causal studies in human biology.

GENOME BIOLOGY (2021)

Article Cell Biology

B0AT1 Amino Acid Transporter Complexed With SARS-CoV-2 Receptor ACE2 Forms a Heterodimer Functional Unit: In Situ Conformation Using Radiation Inactivation Analysis

Bruce R. Stevens et al.

Summary: The study found that the ACE2 receptor of SARS-CoV-2 is expressed at highest levels in the small intestine, potentially affecting the virus tropism in the gastrointestinal tract. Each [ACE2:B(0)AT1] heterodimer pair was shown to constitute a functional unit, providing a basis for further understanding of COVID-19.

FUNCTION (2021)

Article Multidisciplinary Sciences

Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2

Renhong Yan et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Structural basis of receptor recognition by SARS-CoV-2

Jian Shang et al.

NATURE (2020)

Article Medicine, General & Internal

Genomewide Association Study of Severe Covid-19 with Respiratory Failure

David Ellinghaus et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Biochemical Research Methods

Thermophoresis for characterizing biomolecular interaction

Mufarreh Asmari et al.

METHODS (2018)

Article Chemistry, Physical

MicroScale Thermophoresis: Interaction analysis and beyond

Moran Jerabek-Willemsen et al.

JOURNAL OF MOLECULAR STRUCTURE (2014)

Article Biochemical Research Methods

Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

Susanne A. I. Seidel et al.

METHODS (2013)

Review Biochemistry & Molecular Biology

Applications of isothermal titration calorimetry in pure and applied researchusurvey of the literature from 2010

Rajesh Ghai et al.

JOURNAL OF MOLECULAR RECOGNITION (2012)

Review Biochemical Research Methods

Molecular Interaction Studies Using Microscale Thermophoresis

Moran Jerabek-Willemsen et al.

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES (2011)

Review Biochemical Research Methods

Present and future of surface plasmon resonance biosensors

J Homola

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2003)