Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 12, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-023-03777-z
Keywords
Antibiotic resistance; Biocompatibility; Biodegradability; Checkerboard assay; Fractional inhibitory concentration (FIC) index; Minimum inhibitory concentration (MIC)
Categories
Ask authors/readers for more resources
Antimicrobial resistance (AMR) is a global public health issue, and there is a need for new bactericides. This study investigates the antimicrobial activity of Mentha piperita L. leaf extract-produced zinc oxide nanoparticles (ZnONPs). The combination of ZnONPs and Kanamycin (KAN) demonstrates improved antibacterial activity against resistant bacteria and reduces the minimum inhibitory concentration (MIC) of KAN.
Antimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria like Escherichia coli and Staphylococcus aureus were treated. The antibiotics were first screened, and then antibacterial activity was checked by disk diffusion, and MIC of Mp-ZnONPs individually and using Kanamycin (KAN) were determined against these pathogens by broth microdilution method. The synergism between Mp-ZnONPs and KAN was confirmed by checkerboard assay. The MIC showed robust antibacterial activity against the tested pathogens. The combination of KAN and Mp-ZnONPs reduces the MIC of KAN as it efficiently inhibits E. coli's growth, and KAN significantly enhances the antibacterial activity of Mp-ZnONPs. Taken together, Mp-ZnONPs have strong antimicrobial activity, and KAN significantly improves it against the tested pathogens, which would offer an effective, novel, and benign therapeutic methodology to regulate the incidence. The combination of Mp-ZnONPs and KAN would lead to the development of novel bactericides, that could be used in the formulation of pharmaceutical products.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available