Myco-Remediation of Textile Dyes Via Biosorption by Aspergillus tamarii Isolated from Domestic Wastewater

Malachite Green and Reactive Black 5 are commercially used textile dyes which are toxic and recalcitrant in nature and have adverse effect on all living beings coming in its vicinity. Fungal isolate Aspergillus tamarii strain MWWAS8 (GenBank Accession number MZ678772), collected from domestic wastewater treatment plant, Prasanthi Nilayam township was able to decolorize both the dyes. UV-vis spectra showed the reduction of the peaks at their respective wavelength over period of 5 days. Dye decolorization efficiency calculated based on the absorbance recorded using UV-visible spectroscopy showed 97.17% and 96.65% for Malachite Green and Reactive Black 5 respectively at 30 & DEG;C at pH7. Dye samples after treatment did not exhibit inhibitory effect on four bacterial strains namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis which are commonly found in the wastewater treatment plants involved in biological treatment of wastewater. Regeneration assay showed that Aspergillus tamarii had the ability to withstand high concentrations of dyes and regrow upon providing suitable medium. This confirms that the biosorption did not have a detrimental impact on the survival of A. tamarii under the conditions studied.

Automated summary

The fungal isolate Aspergillus tamarii was able to decolorize the toxic and recalcitrant textile dyes Malachite Green and Reactive Black 5. UV-vis spectra showed a reduction in peak intensity over a period of 5 days. The decolorization efficiency was calculated to be 97.17% for Malachite Green and 96.65% for Reactive Black 5 at 30°C and pH 7. The treated dye samples did not inhibit the growth of four common bacterial strains. Regeneration assay confirmed the ability of Aspergillus tamarii to withstand high concentrations of dyes and regrow in suitable medium, indicating no detrimental impact on its survival.