Journal
VETERINARY PARASITOLOGY
Volume 322, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.vetpar.2023.110021
Keywords
Fasciola; Cathepsin L7; Indirect ELISA; Ruminants; Diagnosis
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This study identified a novel member (cathepsin L7) of the cathepsin family from Fasciola spp. and developed a new iELISA for the diagnosis of fasciolosis. The results showed that CL7 iELISA had high sensitivity and specificity in sheep and cattle, and could also detect early infection in buffaloes.
Fasciolosis is a widely distributed zoonosis reported over 81 countries around the world. Good and early diagnostic method is critical in controlling this disease and prevention of injury to the liver and bile ducts. In this study, we identified a novel member (cathepsin L7) of cathepsin family from Fasciola spp.. Firstly, the biological character of CL7 was analyzed according to the information of cathepsin L family, and then rCL7 was expressed and purified, a new iELISA based on CL7 was developed. The results exhibited CL7 iELISA had 100% sensitivity 100% specificity in sheep (cut-off 1.329) and 100% sensitivity 93.75% specificity in cattle (cut-off 0.756). Moreover, anti-Fasciola CL7 antibodies could be detected in early Fasciola gigantica infected buffaloes, as early as 3 week-post-infection (WPI). In conclusion, it is suggested that CL7 with low cost, early detection, good specificity and sensitivity could be used as a candidate antigen for detection of ruminant fasciolosis.
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