Effects of the Alga Aurantiochytrium mangrovei FIKU008-Enriched Artemia on Early Stages of the Green Tiger Shrimp, Penaeus semisulcatus

The first aim of the present study was to determine appropriate artemia enrichment levels by using Aurantiochytrium mangrovei FIKU008 served as DHA enrichment source and secondly to test the effects of enriched-Artemia on growth and survival during the early larval stages(M1-PL7) of Penaeus semisulcatus. In the 1st experiment, four different enrichment levels of AUR (0.0,0.6,0.8 and 1.0g/L) against a control commercial solution (S-presso) were tested for 12/24-hours enrichment while in the 2nd experiment, three levels (0.0, 0.6 and 0.8g/L) of AUR enriched-Artemia were fed to larval stages. At the end of both enrichments, DHA and EPA levels were found to be significantly enhanced by using AUR at 0.6 and 0.8g/L. In the 2nd experiment, fatty acid composition of the PL was significantly affected by AUR and the PL fed with enriched diets showed higher n-3 LC-PUFA accumulation in their tissues. The larvae grown from M1 to PL7 displayed higher survivals and growth in the enriched AUR-groups compared to the control(0.0AUR). In conclusion, this study has demonstrated that the use of AUR as enrichment significantly elevated EPA and DHA levels to 0.6-0.8g/L enrichment periods and the enriched-Artemia at such levels were proven to effectively improve growth and survival of the early larval stages of P. semisulcatus.

Automated summary

The present study aimed to determine appropriate enrichment levels of Aurantiochytrium mangrovei FIKU008 as a DHA source for Artemia and to investigate the effects of enriched-Artemia on the growth and survival of Penaeus semisulcatus larvae during early larval stages (M1-PL7). The first experiment tested four different enrichment levels of AUR (0.0, 0.6, 0.8, and 1.0g/L) against a control solution (S-presso) for 12/24-hours enrichment, while the second experiment fed three levels (0.0, 0.6, and 0.8g/L) of AUR enriched-Artemia to the larvae. The results showed that AUR enrichment significantly increased DHA and EPA levels, and the enriched-Artemia at 0.6-0.8g/L effectively improved the growth and survival of P. semisulcatus during the early larval stages.