Ion current rectification-nanopipette technique for single-cell analysis

Single-cell analysis (SCA) could provide in-depth understanding of cell biology yet is of great challenge. Many attempts have been made to develop novel techniques for SCA. Nanopipette-based ion current rectification (ICR) electroanalysis represents an emerging methodology to combat in this arena. In this review, the working rationale of ICR and its key regulation factors are discussed, followed by the sensing strategies established for SCA using illustrative examples. Future perspectives in this field are also proposed.

Automated summary

Single-cell analysis is a challenging yet important task in cell biology, and various techniques have been developed for this purpose. Nanopipette-based ion current rectification electroanalysis has emerged as a promising method in this field. This review discusses the working principle of this method, its key regulation factors, and presents illustrative examples of sensing strategies for single-cell analysis. Future perspectives in this area are also proposed.