Journal
MOVEMENT DISORDERS
Volume 31, Issue 3, Pages 393-401Publisher
WILEY-BLACKWELL
DOI: 10.1002/mds.26490
Keywords
essential tremor; cerebellum; Purkinje cell; neurodegeneration; pathology
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Funding
- National Institutes of Health (NINDS) [R01 NS042859, R01 NS086736, R01 NS085136, R01 NS088257]
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IntroductionPurkinje cell loss has been documented in some, although not all, postmortem studies of essential tremor. Hence, there is considerable controversy concerning the presence of Purkinje cell loss in this disease. To date, few studies have been performed. MethodsOver the past 8 years, we have assembled 50 prospectively studied cases and 25 age-matched controls; none were reported in our previous large series of 33 essential tremor and 21 controls. In addition to methods used in previous studies, the current study used a random sampling approach to quantify Purkinje cells along the Purkinje cell layer with a mean of 217 sites examined in each specimen, allowing for extensive sampling of the Purkinje cell layer within the section. For the first time, we also quantified the distance between Purkinje cell bodiesa nearest neighbor analysis. ResultsIn the Purkinje cell count data collected from fifteen 100xfields, cases had lower counts than controls in all three counting criteria (cell bodies, nuclei, and nucleoli; all P<0.001). Purkinje cell linear density was also lower in cases than controls (all P<0.001). Purkinje cell linear density obtained by random sampling was similarly lower in cases than controls in all three counting criteria (cell bodies, nuclei, and nucleoli, all P0.005). In agreement with the quantitative Purkinje cell counts, the mean distance from one Purkinje cell body to another Purkinje cell body along the Purkinje cell layer was greater in cases than controls (P=0.002). ConclusionsThese data provide support for the neurodegeneration of cerebellar Purkinje cells in essential tremor. (c) 2016 International Parkinson and Movement Disorder Society
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