Journal
TALANTA
Volume 259, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2023.124566
Keywords
Multiwalled carbon nanotubes; 8-Tetracyanoquinonedimethyl; Resveratrol; Nitric oxide detection; Vascular endothelial cells; Electrochemical sensor
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A convenient and efficient NO sensor was developed using multiwalled carbon nanotubes (MWCNTs)-7,7,8,8-tetracyanoquinodimethan (TCNQ)-polylysine (PLL) modified screen-printed electrode (SPE). The sensor showed good performance for the real-time detection of NO released by human umbilical vein endothelial cells (HUVECs) under different conditions and has potential applications in the diagnosis of biological processes and the screening of drug treatment effects.
Nitric oxide (NO) is an important but short-lived signaling molecule that is released from living cells. Real-time monitoring of NO release is useful for understanding normal cellular physiology and pathology. Herein, a convenient and efficient NO sensor was developed using multiwalled carbon nanotubes (MWCNTs)-7,7,8,8-tetracyanoquinodimethan (TCNQ)-polylysine (PLL) modified screen-printed electrode (SPE). The construction of the sensor (MWCNTs/TCNQ/PLL/SPE) was based on the synergic effect of the good conductivity of TCNQ and the high surface area of MWCNTs. The introduction of the cell-adhesive molecule PLL significantly enhanced the cytocompatibility, resulting in excellent cell attachment and growth. The resulting MWCNTs/TCNQ/PLL/SPE was successfully used for the real-time detection of NO released from living human umbilical vein endothelial cells (HUVECs) cultured on it. The MWCNTs/TCNQ/PLL/SPE was further used to detect NO release from oxidative-injured HUVECs with and without resveratrol to also preliminarily assess the effect of resveratrol against oxidative damage. The sensor developed in this study showed good performance for the real-time detection of NO released by HUVECs under different conditions and has potential applications in the diag-nosis of biological processes and the screening of drug treatment effects.
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