Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 300, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2023.122870
Keywords
Biothiols; Chalcone; Fluorescent probe; Aggregation induced emission; Large Stokes shift
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The homeostasis of biothiols is crucial for organisms' health. A fluorescent probe (7HIN-D) based on a chalcone fluorophore 7HIN was developed for intracellular biothiols detection. The probe exhibited turn-on AIE fluorescence upon the nucleophilic substitution reaction between biothiols and DNBS unit, with good sensitivity and selectivity. It successfully detected endogenous biothiols in living cells due to its excellent performance, biocompatibility, and low cytotoxicity.
The homeostasis of biothiols is closely related to the health of organisms. In view of the important role of biothiols, a fluorescent probe (7HIN-D) for the detection of intracellular biothiols was developed based on a simple chalcone fluorophore 7HIN with ESIPT + AIE characteristics. The probe 7HIN-D was obtained by introducing a biothiols specific DNBS (2,4-dinitrobenzenesulfonyl) unit as a fluorescence quencher to the fluorophore 7HIN. The nucleophilic substitution reaction between biothiols and probe 7HIN-D will release the DNBS unit and the fluorophore 7HIN, which exhibits a turn on AIE fluorescence with a large Stokes shift of 113 nm. The probe 7HIN-D displays high sensitivity and good selectivity to biothiols, and the detection limits value of probe 7HIN-D for GSH, Cys and Hcy were 0.384 mu mol/L, 0.471 mu mol/L and 0.638 mu mol/L, respectively. In addition, the probe has been successfully used for fluorescence detection of endogenous biothiols in living cells due to its excellent performance, good biocompatibility and low cytotoxicity.
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